Immunofluorescence Antibodies | St John's Laboratory

STJ70096 (scale bar: 50 µm; inset: 20 µm) immunostaining of NOS1 neurons in cryosection of a perfusion-fixed (4% PFA) mouse caudate-putamen. HRP-staining with Ni-DAB, after Biotin-SP-anti-goat (IgG) method. Data obtained by Drs. Éva Rumpler and Erik Hrabovszky, Inst, Exp, Med, Budapest, Hungary.

Anti-NOS1 antibody (C-Term) (STJ70096)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Cow

€390.63

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Immunofluorescence analysis of rat-kidney tissue. 1, NOS2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-NOS2 antibody (117-166) (STJ94534)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from RAW264. 7 cells, using iNOS Rabbit pAb (STJ115966) at 1:700 dilution. Raw264. 7 cells were treated by LPS (1 Mu g/ml) at 37 °C for 8 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-NOS2 antibody (948-1153) (STJ115966)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunofluorescence analysis of PC-12 cells using iNOS Rabbit polyclonal antibody (STJ11103799) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NOS2 antibody (974-1143) (STJ11103799)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Rat

€140.63 - €655.63

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Immunofluorescence analysis of human placenta cells using eNOS Rabbit polyclonal antibody (STJ24784) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-NOS3 antibody (1000-1203) (STJ24784)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunofluorescence analysis of Hela cell. 1, NOS3 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-NOS3 antibody (1145-1194) (STJ94539)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunohistochemistry analysis of paraffin-embedded human lymph node (left) and colon cancer (right) tissues with DAB staining using NOS3 monoclonal antibody.

Anti-NOS3 antibody [6H2] (STJ98282)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/ELISA

Reactivity :

Human

€180.00 - €435.00

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Immunofluorescence analysis of U-2 OS cells using NOSTRIN Rabbit polyclonal antibody (STJ118383) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NOSTRIN antibody (270-430) (STJ118383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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STJ73723 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-NOTCH1 antibody (171-182) (STJ73723)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/IHC

Reactivity :

Human/Mouse/Rat/Dog/Cow

€390.63

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Western blot analysis of extracts of Rat brain cells, using Notch1 antibody (STJ29951) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NOTCH1 antibody (2456-2555) (STJ29951)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of extracts of U-251MG cells, using Notch1 antibody (STJ119090) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 300s.

Anti-NOTCH1 antibody (2504-2518) (STJ119090)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-Notch1 antibody [PT0229R] (STJA0039691)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of C6 cells using NOTCH2 Rabbit polyclonal antibody (STJ11105517) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NOTCH2 antibody (1697-1819) (STJ11105517)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunofluorescence analysis of L929 cells using NOTCH2 antibody (STJ29808) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-NOTCH2 antibody (2242-2471) (STJ29808)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Anti-Notch2 antibody [PT0367R] (STJA0039531)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Immunofluorescence analysis of U2OS cells using NOTCH3 Rabbit polyclonal antibody (STJ24789) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NOTCH3 antibody (1520-1640) (STJ24789)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunofluorescence analysis of L929 cells using NOTCH3 Rabbit polyclonal antibody (STJ24788) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-NOTCH3 antibody (751-850) (STJ24788)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

€140.63 - €655.63

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Anti-NOTCH3 antibody [PT1351R] (STJA0040049)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Rat

€0.00

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STJ72523 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing membrane and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-NOTCH4 antibody (1261-1273) (STJ72523)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

€390.63

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Western blot analysis of lysates from Mouse lung, using NOTCH4 Rabbit pAb (STJ110601) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-NOTCH4 antibody (1824-2003) (STJ110601)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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STJ71492 (0. 01µg/ml) staining of Human Breast Cancer lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-NOVA1 antibody (Internal) (STJ71492)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF

Reactivity :

Human/Mouse/Rat/Cow

€390.63

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Immunofluorescence analysis of HT-29 cells using NOX1 Rabbit polyclonal antibody (STJ114195) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NOX1 antibody (200-300) (STJ114195)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

€140.63 - €655.63

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Western blot analysis of 293T Hela VEC KB mouse-kidney 3T3 lysate, antibody was diluted at 2000. Secondary antibody was diluted at 1:20000

Anti-NOX1 antibody (210-260 aa) (STJ98698)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of various cells using Nox3 Polyclonal Antibody

Anti-NOX3 antibody (287-336) (STJ94540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunofluorescence analysis of A-549 cells using NOX5 Rabbit polyclonal antibody (STJ29216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-NOX5 antibody (661-765) (STJ29216)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

€140.63 - €655.63

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Western blot analysis of Jurkat cells using Nox5 Polyclonal Antibody

Anti-NOX5 antibody (677-726) (STJ94541)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-NP/Nucleoprotein antibody [SAA0411] (STJA0019319)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/IF/WB

Reactivity :

Influenza A

€473.13 - €2,040.63

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Anti-NP/Nucleoprotein-strain A/Wilson-Smith/1933 H1N1 antibody [SAA0410] (STJA0019318)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/IF

Reactivity :

Influenza A

€473.13 - €2,040.63

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Immunofluorescence analysis of C6 cells using NPAS2 Rabbit polyclonal antibody (STJ119239) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NPAS2 antibody (45-240) (STJ119239)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using NPAS4 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-NPAS4 antibody (603-652) (STJ94544)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunofluorescence analysis of NIH/3T3 cells using NPB Rabbit polyclonal antibody (STJ119039) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NPB antibody (1-125) (STJ119039)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Immunocytochemistry analysis of Niemann Pick C2 (green) in U87-MG using Niemann Pick C2 antibody, and DAPI (blue).

Anti-NPC2 antibody (20-151 aa) [R07-0C8] (STJA0012496)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

€330.63

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Western blot analysis of extracts of mouse lung, using NPC2 antibody (STJ27366) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NPC2 antibody (20-151) (STJ27366)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of extracts of mouse pancreas, using NPHP1 antibody (STJ28757) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NPHP1 antibody (463-732) (STJ28757)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various lysates using NPHP4 Rabbit pAb (STJ116333) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-NPHP4 antibody (1247-1426) (STJ116333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunohistochemistry analysis of paraffin-embedded human brain, using NPHP4 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-NPHP4 antibody (877-926) (STJ94402)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunohistochemistry analysis of Nephrin antibody in paraffin-embedded human brain tissue.

Anti-NPHS1 antibody (843-892) (STJ94401)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunofluorescence analysis of U2OS cells using NPLOC4 Rabbit polyclonal antibody (STJ111178) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-NPLOC4 antibody (1-270) (STJ111178)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various cells using Nucleophosmin Polyclonal Antibody diluted at 1：2000

Anti-NPM1 antibody (1-50) (STJ94573)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€60.00 - €315.00

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Hela whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Nucleophosmin (ABT210) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 33kDaObserved band size: 33kDa

Anti-NPM1 antibody (100-200 aa) [ABT210] (STJ197178)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

€217.50 - €315.00

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Anti-NPM1 antibody (100-200 aa) [ABT358] (STJ197179)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

€217.50 - €315.00

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Western blot analysis of various cells using NPM Polyclonal Antibody

Anti-NPM1 antibody (201-250) (STJ94546)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunofluorescence analysis of U2OS cells using NPM1 Rabbit polyclonal antibody (STJ119961) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-NPM1 antibody (9-122) (STJ119961)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis using B23 monoclonal antibody against HeLa, Jurkat, 3T3 cell lysate.

Anti-NPM1 antibody (STJ98470)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat/Cow/Pig/Rabbit

€180.00 - €435.00

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Western blot analysis using B23 monoclonal antibody against SMMC-7721 (1) , HepG2 (2) , HeLa (3) and HEK293 (4) cell lysate.

Anti-NPM1 antibody [7H10B9] (STJ97858)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

€180.00 - €435.00

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Confocal imaging of paraffin-embedded mouse heart using NPPA Rabbit monoclonal antibody (STJ11104694, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-NPPA antibody (1-100) [S4694RM] (STJ11104694)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€161.88 - €718.13

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Western blot analysis of various lysates using NPPA Rabbit polyclonal antibody (STJ24798) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-NPPA antibody (26-151) (STJ24798)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-NPPB antibody (100-150) (STJ502173)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

€534.38

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