• Immunofluorescence analysis of A549 cells using LIG1 antibody (STJ24405).
  • Immunofluorescence analysis of MCF-7 cells using LIG1 antibody (STJ24405). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using LIG1 Rabbit polyclonal antibody (STJ24405) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-LIG1 antibody (565-790) (STJ24405)

SKU:
STJ24405

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LIG1 (565-790) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LIG1
Gene ID: 3978
Uniprot ID: DNLI1_HUMAN
Immunogen Region: 565-790
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 565-790 of human LIG1 (NP_000225.1).
Immunogen Sequence: CEYKYDGQRAQIHALEGGEV KIFSRNQEDNTGKYPDIISR IPKIKLPSVTSFILDTEAVA WDREKKQIQPFQVLTTRKRK EVDASEIQVQVCLYAFDLIY LNGESLVREPLSRRRQLLRE NFVETEGEFVFATSLDTKDI EQIAEFLEQSVKDSCEGLMV KTLDVDATYEIAKRSHNWLK LKKDYLDGVGDTLDLVVIGA YLGRGKRAGRYGGFLLASYD EDSEEL
Function DNA ligase that seals nicks in double-stranded during DNA repair. Also involved in DNA replication and DNA recombination.
Protein Name Dna Ligase 1
Dna Ligase I
Polydeoxyribonucleotide Synthase Atp 1
Database Links Reactome: R-HSA-110362
Reactome: R-HSA-162594
Reactome: R-HSA-174414
Reactome: R-HSA-5358565
Reactome: R-HSA-5358606
Reactome: R-HSA-5651801
Reactome: R-HSA-5696397
Reactome: R-HSA-6782210
Reactome: R-HSA-69183
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Dna Ligase 1 antibody
Anti-Dna Ligase I antibody
Anti-Polydeoxyribonucleotide Synthase Atp 1 antibody
Anti-LIG1 antibody

Information sourced from Uniprot.org

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