• STJ72007 (0. 03µg/ml) staining of Human Cerebellum (A) and Frontal Cortex (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ72007 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-LIGHT/CD258 antibody (Internal) (STJ72007)

SKU:
STJ72007-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-LIGHT/CD258 (Internal) is suitable for use in ELISA, Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.03-0.1µg/ml
IF-Strong expression of the protein seen in the cytoplasm and membrane of HeLa cells. 10µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: TNFSF14
Gene ID: 8740
Uniprot ID: TNF14_HUMAN
Immunogen Region: Internal
Accession Number: NP_003798.2; NP_742011.1
Specificity: This antibody is expected to recognize both reported isoforms (NP_003798.2; NP_742011.1).
Immunogen Sequence: HEVNPAAHLTGANS
Post Translational Modifications N-glycosylated. The soluble form of isoform 1 derives from the membrane form by proteolytic processing.
Function Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Acts as a ligand for TNFRSF14/HVEM. Upon binding to TNFRSF14/HVEM, delivers costimulatory signals to T cells, leading to T cell proliferation and IFNG production.
Protein Name Tumor Necrosis Factor Ligand Superfamily Member 14
Herpes Virus Entry Mediator Ligand
Hvem-L
Herpesvirus Entry Mediator Ligand
Cd Antigen Cd258 Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 14 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 14 - Soluble Form
Database Links Reactome: R-HSA-5668541
Reactome: R-HSA-5669034
Reactome: R-HSA-5676594
Cellular Localisation Tumor Necrosis Factor Ligand Superfamily Member 14
Membrane Form: Cell Membrane
Single-Pass Type Ii Membrane Protein
Soluble Form: Secreted
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Tumor Necrosis Factor Ligand Superfamily Member 14 antibody
Anti-Herpes Virus Entry Mediator Ligand antibody
Anti-Hvem-L antibody
Anti-Herpesvirus Entry Mediator Ligand antibody
Anti-Cd Antigen Cd258 Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 14 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 14 - Soluble Form antibody
Anti-TNFSF14 antibody
Anti-HVEML antibody
Anti-LIGHT antibody
Anti-UNQ391 antibody
Anti-PRO726 antibody

Information sourced from Uniprot.org

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