Immunofluorescence Antibodies | St John's Laboratory

Immunofluorescence analysis of paraffin-embedded Mouse kidney using MIOX Rabbit polyclonal antibody (STJ116619) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MIOX antibody (1-285) (STJ116619)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using AQP0 Polyclonal Antibody

Anti-MIP antibody (95-144 aa) (STJ91651)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence – anti-Miranda antibody in fly cells at 1:100 dilution; InsGAL4 UASCD8 GFP wandering L3; cells were fixed with 4% of PFA

Anti-Miranda antibody (220 aa N-Term) (STJ140173)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

D.melanogaster

£135.50 - £394.50

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Immunofluorescence – anti-Miranda antibody in fly cells at 1/50 dilution; cells were fixed with 4% of PFA

Anti-Miranda antibody (220 aa N-Term) (STJ140260)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IF

Reactivity :

D.melanogaster

£135.50 - £394.50

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Western blot analysis of hela cells using primary antibody diluted at 1:500. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-MITF antibody (151-200) (STJ94134)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Confocal imaging of HeLa cells using MITF Rabbit monoclonal antibody (STJ11101379, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-MITF antibody (387-509) [S9MR] (STJ11101379)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-mKalama antibody (STJ140290)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

GFP

£135.50 - £394.50

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Immunofluorescence analysis of paraffin-embedded Rat spleen using Ki67 Rabbit monoclonal antibody (STJ11104589) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MKI67 antibody (1200-1300) [S4589RM] (STJ11104589)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Whole cell lysates were separated by 6% SDS-PAGE, and the membrane was blotted with anti-Ki-67 (ABT001) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: MCF7Predicted band size: 359kDaObserved band size: 359kDa

Anti-MKI67 antibody (1200-1400 aa) [ABT001] (STJ197092)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£174.00 - £252.00

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Immunofluorescence analysis of paraffin-embedded Rat spleen tissue using Mouse Mki67 Rabbit polyclonal antibody (STJ11105641) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-Mki67 antibody (1816-2163) (STJ11105641)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of Hela cell. 1, Ki-67 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HSP70 monoclonal antibody (3G10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-MKI67 antibody (3207-3256 aa) (STJ93832)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human

£48.00 - £252.00

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Immunofluorescence analysis of HeLa cells using Ki67 Rabbit polyclonal antibody (STJ119231) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MKI67 antibody (460-680) (STJ119231)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells using Ki67 Rabbit polyclonal antibody (STJ113112) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MKI67 antibody (700-800) (STJ113112)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of Hela cell. 1, Annexin VI Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-MKI67 antibody [4A8] (STJ96966)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£48.00 - £252.00

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Fluorescence multiplex immunohistochemical analysis of Human tonsil tissue (formalin-fixed paraffin-embedded section). The immunostaining was performed by Pentuple-Fluorescence kit. CK-pan mouse monoclonal antibody (STJ197095 green), Ki-67 rabbit monoclonal antibody (STJA0006295) red, Iba 1 mouse monoclonal antibody (purple), CD8 a mouse monoclonal antibody (cyan), CD20 mouse monoclonal antibody (yellow), The section was incubated in 5 rounds of staining; sequentially for Anti-antibodies; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (Excilone).

Anti-MKI67 antibody [ABT104R] (STJA0006295)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human

£144.00 - £234.00

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Western blot analysis of various lysates using MKL1 Rabbit pAb (STJ110802) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-MKL1 antibody (1-280) (STJ110802)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U-2 OS cells using MKL1 Polyclonal Antibody (STJ116144) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-MKL1 antibody (1-280) (STJ116144)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using Mnk1 Polyclonal Antibody diluted at 1：500

Anti-MKNK1 antibody (111-160) (STJ94170)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of NIH-3T3 cells using MKNK1 Rabbit polyclonal antibody (STJ11100383) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-MKNK1 antibody (374-465) (STJ11100383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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STJ72567 (1µg/ml) staining of Mouse fetal (A) and adult (B) Brain lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-MKRN1 antibody (105-118) (STJ72567)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Cow

£312.50

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Western blot analysis of extracts of various cell lines, using MKS1 antibody (STJ26874) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MKS1 antibody (150-300) (STJ26874)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded Human melanoma using MLANA antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-MLANA antibody (2-33 aa) [R08-8B5] (STJA0014658)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human

£264.50

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Western blot analysis of 3T3 cells using Melan-A Polyclonal Antibody

Anti-MLANA antibody (41-90 aa) (STJ94093)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of A375 cells using Melan-A/MART-1 Rabbit polyclonal antibody (STJ28212) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-MLANA antibody (48-118) (STJ28212)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Human malignant melanoma tissue was stained with Anti-Melan-A (ABT-Mart1) Antibody

Anti-MLANA antibody (50-118 aa) [ABT-Mart1] (STJ197030)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Anti-MLANA antibody (50-118 aa) [ABT193] (STJ197169)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Western blot analysis of various lysates using MLF1 Rabbit pAb (STJ110318) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-MLF1 antibody (1-268) (STJ110318)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of A549 cells using MLF1 antibody (STJ115292).

Anti-MLF1 antibody (1-268) (STJ115292)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis using MLH1 monoclonal antibody against HeLa (1) , MCF-7 (2) and A549 (3) , Jurkat (4) , 2R75 (5) and COS (6) cell lysate.

Anti-MLH1 antibody (381-483 aa) [4C9C7] (STJ98245)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

£144.00 - £348.00

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Various whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-MLH1 (ABT-MLH1) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: K562 Lane 2: HEK293 Lane 3: HelaPredicted band size: 84kDaObserved band size: 84kDa

Anti-MLH1 antibody (400-500 aa) [ABT-MLH1] (STJ196792)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Anti-MLH1 antibody [SM0450] (STJA0010450)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Anti-MLH1 antibody [SM5040] (STJA0005040)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-MLH3 antibody (521-570) (STJ94146)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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STJ70213 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-MLH3 antibody (C-Term) (STJ70213)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human

£312.50

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Western blot analysis of various lysates using MLKL Rabbit polyclonal antibody (STJ29918) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MLKL antibody (1-62) (STJ29918)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using MLKL Rabbit polyclonal antibody (STJ119442) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MLKL antibody (369-471) (STJ119442)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded Human Tonsil Tissue using MLKL Mouse mAb diluted at 1:200.

Anti-MLKL antibody [8H7] (STJ97792)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, AF-10 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). COX IV monoclonal antibody (6C8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-MLLT10 antibody (211-260 aa) (STJ91504)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of U-2 OS cells using MLPH antibody (STJ28739) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-MLPH antibody (301-600) (STJ28739)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using MRLC2 Polyclonal Antibody

Anti-MLRN antibody (3-52) (STJ94204)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of U2OS cells using MLST8 antibody (STJ115560).

Anti-MLST8 antibody (1-326) (STJ115560)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-MLX antibody (111-160) (STJ94156)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-mMaroon antibody (STJ140272)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

Far-RFP

£135.50 - £394.50

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A) WB of STJ501899 with SKnSH cells. 1:500 antibody dilution in buffer. B) IHC with STJ501894 with SKnSH cells. C) IHC of STJ501894 Hoechst staining. 1:200 antibody dilution in buffer.

Anti-MME antibody (100-150) (STJ501894)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-MME antibody (100-150) (STJ501899)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-MME antibody (100-150) {Biotin} (STJ501901)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-MME antibody (100-150) {Biotin} (STJ501911)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-MME antibody (100-150) {FITC} (STJ501902)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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