• Western blot analysis of extracts of various cell lines, using GHRHR antibody (STJ110719) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 15s.
  • Immunofluorescence analysis of rat pituitary gland cells using GHRHR Rabbit polyclonal antibody (STJ110719) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat pituitary gland cells using GHRHR rabbit polyclonal antibody (STJ110719) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using GHRHR Rabbit polyclonal antibody (STJ110719) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-GHRHR antibody (1-130) (STJ110719)

SKU:
STJ110719

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GHRHR (1-130) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GHRHR
Gene ID: 2692
Uniprot ID: GHRHR_HUMAN
Immunogen Region: 1-130
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human GHRHR (NP_000814.2).
Immunogen Sequence: MDRRMWGAHVFCVLSPLPTV LGHMHPECDFITQLREDESA CLQAAEEMPNTTLGCPATWD GLLCWPTAGSGEWVTLPCPD FFSHFSSESGAVKRDCTITG WSEPFPPYPVACPVPLELLA EEESYFSTVK
Tissue Specificity Pituitary gland.
Function Receptor for GRF, coupled to G proteins which activate adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone gene transcription and growth hormone secretion.
Protein Name Growth Hormone-Releasing Hormone Receptor
Ghrh Receptor
Growth Hormone-Releasing Factor Receptor
Grf Receptor
Grfr
Database Links Reactome: R-HSA-418555
Reactome: R-HSA-420092
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Growth Hormone-Releasing Hormone Receptor antibody
Anti-Ghrh Receptor antibody
Anti-Growth Hormone-Releasing Factor Receptor antibody
Anti-Grf Receptor antibody
Anti-Grfr antibody
Anti-GHRHR antibody

Information sourced from Uniprot.org

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