• Western blot analysis of lysate from 293 cells, using Ghrelin antibody.
  • Immunohistochemistry analysis of Ghrelin antibody in paraffin-embedded human lung carcinoma tissue.
  • Western blot analysis of 293 cells using Ghrelin Polyclonal Antibody
  • Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of various cells using Ghrelin Polyclonal Antibody

Anti-GHRL antibody (47-96 aa) (STJ93265)

SKU:
STJ93265

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Appetite-regulating hormone (47-96 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-300
ELISA 1:20000
IF 1:100-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GHRL
Gene ID: 51738
Uniprot ID: GHRL_HUMAN
Immunogen Region: 47-96 aa
Specificity: Ghrelin Polyclonal Antibody detects endogenous levels of Ghrelin protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Ghrelin at the amino acid range 47-96
Post Translational Modifications O-octanoylated by GOAT/MBOAT4. O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid. Also O-acetylated and O-butyrylated on Ser-26 to minor levels. Amidation of Leu-98 is essential for obestatin activity.
Function Ghrelin-27: Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation. Ghrelin-28: Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation. Obestatin: May be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility.
Protein Name Appetite-Regulating Hormone
Growth Hormone Secretagogue
Growth Hormone-Releasing Peptide
Motilin-Related Peptide
Protein M46 Cleaved Into - Ghrelin-27 - Ghrelin-28
Ghrelin - Obestatin
Database Links Reactome: R-HSA-375276 Q9UBU3-1
Reactome: R-HSA-416476 Q9UBU3-1
Reactome: R-HSA-422085
Cellular Localisation Secreted
Alternative Antibody Names Anti-Appetite-Regulating Hormone antibody
Anti-Growth Hormone Secretagogue antibody
Anti-Growth Hormone-Releasing Peptide antibody
Anti-Motilin-Related Peptide antibody
Anti-Protein M46 Cleaved Into - Ghrelin-27 - Ghrelin-28 antibody
Anti-Ghrelin - Obestatin antibody
Anti-GHRL antibody
Anti-MTLRP antibody
Anti-UNQ524 antibody
Anti-PRO1066 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance