Immunocytochemistry Antibodies | St John's Laboratory

Western blot analysis of extracts of NCI-H460 cells, using IL18R1/IL-18 R alpha antibody (STJ24172) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IL18R1 antibody (19-320) (STJ24172)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of THP-1 cells using IL1 Beta Rabbit polyclonal antibody (STJ113778) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IL1B antibody (1-269) (STJ113778)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

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Western blot analysis of various lysates using IL1 Beta Rabbit pAb (STJ24174) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-IL1B antibody (1-269) (STJ24174)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of THP-1 cells, using IL1 Beta antibody (STJ11101739) at 1:1000 dilution. THP-1 cells were treated by LPS (1 Mu g/mL) at 37 °C for 8 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-IL1B antibody (117-269) [S9MR] (STJ11101739)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

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Western blot analysis of recombinant mouse IL-1 beta protein, using IL1 Beta Rabbit monoclonal antibody (STJ11104780) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10ng/5ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-IL1Beta antibody (118-269) [S4780RM] (STJ11104780)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse

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Western blot analysis of various lysates using IL36R Rabbit polyclonal antibody (STJ112130) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-IL1RL2 antibody (1-116) (STJ112130)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

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Confocal imaging of mouse tail ear using IL1RA Rabbit monoclonal antibody (STJ11101277, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-IL1RN antibody (100-177) [S7MR] (STJ11101277)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of extracts of A-431 cells, using IL1RA antibody (STJ24179) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IL1RN antibody (24-177) (STJ24179)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of NIH/3T3 cells using IL1RN Rabbit polyclonal antibody (STJ11104175) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-IL1RN antibody (27-178) (STJ11104175)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Anti-IL2 antibody [SRM15021] (STJ11109751)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

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Western blot analysis of extracts of various cell lines, using (STJ29315) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-IL21 antibody (25-162) (STJ29315)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of extracts of normal 293T cells and 293T transfected with IL22 using IL22 Rabbit mAb (STJ11107003) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 45s.

Anti-IL22 antibody (34-179 aa) [SRM5159] (STJ11107003)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of HL-60, using IL22 antibody (STJ27972) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-IL22 antibody (34-179) (STJ27972)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of L929 cells using IL23R Rabbit polyclonal antibody (STJ24181) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-IL23R antibody (510-629) (STJ24181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with 17E/IL25 using IL25 Rabbit pAb (STJ110551) at 1:2000 dilution. <br/> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/> Lysates/ proteins: 25 Mu g per lane. <br/> Blocking buffer: 3 % nonfat dry milk in TBST. <br/> Detection: ECL Basic Kit <br/> Exposure time: 90s.

Anti-IL25 antibody (33-177) (STJ110551)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of extracts of various cell lines, using CD25 antibody (STJ24183) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-IL2RA antibody (153-167) (STJ24183)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/FC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-IL33 antibody (109-270 aa) [SRM5370] (STJ11107280)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

$174.83 - $775.58

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Anti-IL33 antibody [SRM14549] (STJ11109279)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IF-P/IHC-P/ELISA

Reactivity :

Human

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Western blot analysis of extracts of various cell lines, using IL36G antibody (STJ112204) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-IL36G antibody (1-193) (STJ112204)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

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Immunofluorescence analysis of paraffin-embedded Human breast cancer tissue using IL4 Rabbit polyclonal antibody (STJ27616) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-IL4 antibody (25-153) (STJ27616)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

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Western blot analysis of various lysates using IL5 Rabbit pAb (STJ110003) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit Exposure time: 15s.

Anti-IL5 antibody (20-134) (STJ110003)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of recombinant Human IL-5 Protein, using Human IL-5 Rabbit polyclonal antibody (STJ11104218) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10ng/5ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 8s.

Anti-IL5 antibody (20-134) (STJ11104218)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

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Immunofluorescence analysis of U-87MG cells using IL6 Rabbit monoclonal antibody (STJ11104247) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-IL6 antibody (25-211) [S4247RM] (STJ11104247)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human/Rat

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Immunofluorescence analysis of NIH/3T3 cells using IL6 Rabbit polyclonal antibody (STJ110936) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IL6 antibody (30-212) (STJ110936)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of U2OS cells using IL6ST Rabbit polyclonal antibody (STJ11100009) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IL6ST antibody (751-918) (STJ11100009)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of extracts of various cell lines, using CD127/IL7R rabbit monoclonal antibody (STJ11101386) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IL7R antibody (300-400) [S6MR] (STJ11101386)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from THP-1 cells using IL8 Rabbit mAb (STJ11107026) at 1:1000 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37C for overnight and LPS (1 Mu g/ml) at 37C for 6 hours. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.

Anti-IL8 antibody (28-99 aa) [SRM5168] (STJ11107026)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$174.83 - $775.58

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Anti-IL8 antibody [SRM14770] (STJ11109500)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/FC/ELISA

Reactivity :

Human

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Anti-IL9 antibody {ABflo® 594} (STJ11108645)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/FC

Reactivity :

Human

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g ILF3 antibody (STJ24197). Western blot was performed from the immunoprecipitate using ILF3 antibody (STJ24197) at a dilution of 1:1000.

Anti-ILF3 antibody (1-270) (STJ24197)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Confocal imaging of 293F cells transfected with ILT4 using ILT4 Rabbit mAb (STJ11107370, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-ILT4 antibody (22-461 aa) [SRM5456] (STJ11107370)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

$174.83 - $775.58

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Immunofluorescence analysis of NIH/3T3 cells using IMMP2L Rabbit polyclonal antibody (STJ11105236) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IMMP2L antibody (38-175) (STJ11105236)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

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Confocal immunofluorescence analysis of U2OS cells using IMMT Rabbit polyclonal antibody (STJ24199) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-IMMT antibody (120-270) (STJ24199)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-IMMT antibody [M027] (STJA0005467)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human

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Immunofluorescence analysis of A549 cells using IMPA1 antibody (STJ28464). Blue: DAPI for nuclear staining.

Anti-IMPA1 antibody (1-277) (STJ28464)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various lysates using IMPDH1 Rabbit pAb (STJ111723) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-IMPDH1 antibody (434-563) (STJ111723)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various lysates, using [KO Validated] IMPDH2 Rabbit mAb (STJ11102455) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-IMPDH2 antibody (100-300) [S5MR] (STJ11102455)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Immunocytochemistry analysis of IMPDH2 (green) in Hela using IMPDH2 antibody, and DAPI (blue).

Anti-IMPDH2 antibody (140-154 aa) [R06-1B8] (STJA0012430)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

$357.08

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Immunofluorescence analysis of HeLa cells using IMPDH2 antibody (STJ27303).

Anti-IMPDH2 antibody (215-514) (STJ27303)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-Importin Alpha 5/KPNA1 antibody [SRM14717] (STJ11109447)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of paraffin-embedded mouse brain using Alpha Internexin Rabbit monoclonal antibody (STJ11101907) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-INA antibody (350-450) [S7MR] (STJ11101907)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot with Anti-inaD Antibody (STJ501452) with peptide on at 1:1000 dilution in buffer.

Anti-inaD antibody (659-674) (STJ501452)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

D.melanogaster

$577.13

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Anti-inaD antibody (659-674) {Biotin} (STJ501453)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

D.melanogaster

$713.48

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Anti-inaD antibody (659-674) {FITC} (STJ501454)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

D.melanogaster

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Immunofluorescence analysis of MCF-7 cells using ING5 antibody (STJ29427). Blue: DAPI for nuclear staining.

Anti-ING5 antibody (1-220) (STJ29427)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

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Immunohistochemistry of baboon liver with Anti-INHA primary antibody (STJ501455) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-INHA antibody (1-50) (STJ501455)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human/Monkey/Mouse/Rat

$577.13

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Anti-INHA antibody (1-50) {Biotin} (STJ501462)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-INHA antibody (1-50) {FITC} (STJ501463)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human/Monkey/Mouse/Rat

$713.48

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