Anti-ENO1 antibody (1-434) (STJ23538)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ENO1 (1-434) antibody for use in WB, IHC-P, IF, ICC, IP and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000IHC-P:1:50-1:200IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your speci |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ENO1 |
| Gene ID | 2023 |
| Uniprot ID | ENOA_HUMAN |
| Immunogen Region | 1-434 |
| Immunogen Sequence | MSILKIHAREIFDSRGNPTV EVDLFTSKGLFRAAVPSGAS TGIYEALELRDNDKTRYMGK GVSKAVEHINKTIAPALVSK KLNVTEQEKIDKLMIEMDGT ENKSKFGANAILGVSLAVCK AGAVEKGVPLYRHIADLAGN SEVILPVPAFNVINGGSHAG NKLAMQEFMILPVGAANFRE AMRIGAEVYHNLKNVIKEKY GKDATNVGDEGGFAPNILEN KEGLELLKTAIGKAGYTDK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-434 of human ENO1 (NP_001419.1). |
Additional Info
| Tissue Specificity | The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. |
| Post Translational Modifications | ISGylated. Lysine 2-hydroxyisobutyrylation (Khib) by p300/EP300 activates the phosphopyruvate hydratase activity. |
| Function | Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. Isoform MBP-1: Binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor. |
| Protein Name | Alpha-Enolase2-Phospho-D-Glycerate Hydro-LyaseC-Myc Promoter-Binding ProteinEnolase 1Mbp-1Mpb-1Non-Neural EnolaseNnePhosphopyruvate HydratasePlasminogen-Binding Protein |
| Database Links | Reactome: R-HSA-70171Reactome: R-HSA-70263Reactome: R-HSA-9636667 |
| Cellular Localisation | CytoplasmCell MembraneMyofibrilSarcomereM LineCan Translocate To The Plasma Membrane In Either The Homodimeric (Alpha/Alpha) Or Heterodimeric (Alpha/Gamma) FormEno1 Is Localized To The M LineIsoform Mbp-1: Nucleus |
| Alternative Antibody Names | Anti-Alpha-Enolase antibodyAnti-2-Phospho-D-Glycerate Hydro-Lyase antibodyAnti-C-Myc Promoter-Binding Protein antibodyAnti-Enolase 1 antibodyAnti-Mbp-1 antibodyAnti-Mpb-1 antibodyAnti-Non-Neural Enolase antibodyAnti-Nne antibodyAnti-Phosphopyruvate Hydratase antibodyAnti-Plasminogen-Binding Protein antibodyAnti-ENO1 antibodyAnti-ENO1L1 antibodyAnti-MBPB1 antibodyAnti-MPB1 antibody |
Information sourced from Uniprot.org