• Immunofluorescence analysis of paraffin-embedded mouse testis using ELSPBP1 Rabbit polyclonal antibody (STJ118327) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded rat testis using ELSPBP1 Rabbit polyclonal antibody (STJ118327) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ELSPBP1 Rabbit polyclonal antibody (STJ118327) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ELSPBP1 antibody (110-223) (STJ118327)

SKU:
STJ118327

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Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ELSPBP1 (110-223) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ELSPBP1
Gene ID: 64100
Uniprot ID: ESPB1_HUMAN
Immunogen Region: 110-223
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 110-223 of human ELSPBP1 (NP_071425.3).
Immunogen Sequence: QQWKFCETNEYGGNSLRKPC IFPSIYRNNVVSDCMEDESN KLWCPTTENMDKDGKWSFCA DTRISALVPGFPCHFPFNYK NKNYFNCTNEGSKENLVWCA TSYNYDQDHTWVYC
Tissue Specificity Detected in cauda epididymidal fluid and on sperm membrane (at protein level).
Post Translational Modifications N-glycosylated.
Function Binds to spermatozoa upon ejaculation and may play a role in sperm capacitation. Has phosphorylcholine-binding activity.
Protein Name Epididymal Sperm-Binding Protein 1
Epididymal Secretory Protein 12
He12
Cellular Localisation Secreted
Alternative Antibody Names Anti-Epididymal Sperm-Binding Protein 1 antibody
Anti-Epididymal Secretory Protein 12 antibody
Anti-He12 antibody
Anti-ELSPBP1 antibody
Anti-E12 antibody

Information sourced from Uniprot.org

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