• Immunofluorescence analysis of paraffin-embedded human placenta using ENTPD1/CD39 Rabbit polyclonal antibody (STJ23545) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of THP-1 cells using ENTPD1/CD39 Rabbit polyclonal antibody (STJ23545) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Raw264. 7 cells using ENTPD1/CD39 Rabbit polyclonal antibody (STJ23545) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of ENTPD1/CD39 in paraffin-embedded human placenta using ENTPD1/CD39 Rabbit polyclonal antibody (STJ23545) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ENTPD1/CD39 in paraffin-embedded human liver using ENTPD1/CD39 Rabbit polyclonal antibody (STJ23545) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using ENTPD1/CD39 Rabbit polyclonal antibody (STJ23545) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ENTPD1 antibody (1-180) (STJ23545)

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STJ23545

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ENTPD1/CD39 (1-180) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ENTPD1
Gene ID: 953
Uniprot ID: ENTP1_HUMAN
Immunogen Region: 1-180
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ENTPD1/CD39 (NP_001157655.1).
Immunogen Sequence: MESEELADRVLDVVERSLSN YPFDFQGARIITGQEEGAYG WITINYLLGKFSQKTRWFSI VPYETNNQETFGALDLGGAS TQVTFVPQNQTIESPDNALQ FRLYGKDYNVYTHSFLCYGK DQALWQKLAKDIQVASNEIL RDPCFHPGYKKVVNVSDLYK TPCTKRFEMTLPFQQFEIQG
Tissue Specificity Expressed primarily on activated lymphoid cells. Also expressed in endothelial tissues. Isoform 1 and isoform 3 are present in both placenta and umbilical vein, whereas isoform 2 is present in placenta only.
Post Translational Modifications The N-terminus is blocked. Palmitoylated in the N-terminal part.
Function In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well.
Protein Name Ectonucleoside Triphosphate Diphosphohydrolase 1
Ntpdase 1
Ecto-Atp Diphosphohydrolase 1
Ecto-Atpdase 1
Ecto-Atpase 1
Ecto-Apyrase
Lymphoid Cell Activation Antigen
Cd Antigen Cd39
Database Links Reactome: R-HSA-8850843
Reactome: R-HSA-9660826
Cellular Localisation Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Ectonucleoside Triphosphate Diphosphohydrolase 1 antibody
Anti-Ntpdase 1 antibody
Anti-Ecto-Atp Diphosphohydrolase 1 antibody
Anti-Ecto-Atpdase 1 antibody
Anti-Ecto-Atpase 1 antibody
Anti-Ecto-Apyrase antibody
Anti-Lymphoid Cell Activation Antigen antibody
Anti-Cd Antigen Cd39 antibody
Anti-ENTPD1 antibody
Anti-CD39 antibody

Information sourced from Uniprot.org

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