Anti-ENAH antibody (412-591) (STJ119918)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ENAH (412-591) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ENAH |
| Gene ID | 55740 |
| Uniprot ID | ENAH_HUMAN |
| Immunogen Region | 412-591 |
| Immunogen Sequence | FPSGGNAIGVNSASSKTDTG RGNGPLPLGGSGLMEEMSAL LARRRRIAEKGSTIETEQKE DKGEDSEPVTSKASSTSTPE PTRKPWERTNTMNGSKSPVI SRRDSPRKNQIVFDNRSYDS LHRPKSTPLSQPSANGVQTE GLDYDRLKQDILDEMRKELT KLKEELIDAIRQELSKSNTA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 412-591 of human ENAH (NP_001008493.1). |
Additional Info
| Tissue Specificity | Expressed in myoepithelia of parotid, breast, bronchial glands and sweat glands. Expressed in colon-rectum muscolaris mucosae epithelium, pancreas acinar ductal epithelium, endometrium epithelium, prostate fibromuscolar stroma and placenta vascular media. Overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions. |
| Post Translational Modifications | NTN1-induced PKA phosphorylation on Ser-265 directly parallels the formation of filopodial protrusions. |
| Function | Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. |
| Protein Name | Protein Enabled Homolog |
| Database Links | Reactome: R-HSA-202433Reactome: R-HSA-376176 |
| Cellular Localisation | CytoplasmCytoskeletonCell ProjectionLamellipodiumFilopodiumSynapseCell JunctionFocal AdhesionTargeted To The Leading Edge Of Lamellipodia And Filopodia By Mrl Family MembersColocalizes At Filopodial Tips With A Number Of Other Proteins Including Vinculin And ZyxlinColocalizes With N-Wasp At The Leading EdgeColocalizes With Gphn And Pfn At Synapses |
| Alternative Antibody Names | Anti-Protein Enabled Homolog antibodyAnti-ENAH antibodyAnti-MENA antibody |
Information sourced from Uniprot.org