• Immunofluorescence analysis of U-2 OS cells using ENAH Rabbit polyclonal antibody (STJ119918) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using ENAH Rabbit polyclonal antibody (STJ119918) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ENAH Rabbit polyclonal antibody (STJ119918) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-ENAH antibody (412-591) (STJ119918)

SKU:
STJ119918

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ENAH (412-591) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ENAH
Gene ID: 55740
Uniprot ID: ENAH_HUMAN
Immunogen Region: 412-591
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 412-591 of human ENAH (NP_001008493.1).
Immunogen Sequence: FPSGGNAIGVNSASSKTDTG RGNGPLPLGGSGLMEEMSAL LARRRRIAEKGSTIETEQKE DKGEDSEPVTSKASSTSTPE PTRKPWERTNTMNGSKSPVI SRRDSPRKNQIVFDNRSYDS LHRPKSTPLSQPSANGVQTE GLDYDRLKQDILDEMRKELT KLKEELIDAIRQELSKSNTA
Tissue Specificity Expressed in myoepithelia of parotid, breast, bronchial glands and sweat glands. Expressed in colon-rectum muscolaris mucosae epithelium, pancreas acinar ductal epithelium, endometrium epithelium, prostate fibromuscolar stroma and placenta vascular media. Overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions.
Post Translational Modifications NTN1-induced PKA phosphorylation on Ser-265 directly parallels the formation of filopodial protrusions.
Function Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation.
Protein Name Protein Enabled Homolog
Database Links Reactome: R-HSA-202433
Reactome: R-HSA-376176
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Lamellipodium
Filopodium
Synapse
Cell Junction
Focal Adhesion
Targeted To The Leading Edge Of Lamellipodia And Filopodia By Mrl Family Members
Colocalizes At Filopodial Tips With A Number Of Other Proteins Including Vinculin And Zyxlin
Colocalizes With N-Wasp At The Leading Edge
Colocalizes With Gphn And Pfn At Synapses
Alternative Antibody Names Anti-Protein Enabled Homolog antibody
Anti-ENAH antibody
Anti-MENA antibody

Information sourced from Uniprot.org

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