• Immunofluorescence analysis of L929 cells using EML4 Rabbit polyclonal antibody (STJ118557) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of EML4 in paraffin-embedded Human liver cancer using EML4 Rabbit polyclonal antibody (STJ118557) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of EML4 in paraffin-embedded Rat kidney using EML4 Rabbit polyclonal antibody (STJ118557) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of EML4 in paraffin-embedded Mouse kidney using EML4 Rabbit polyclonal antibody (STJ118557) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from HepG2 cells, using EML4 Rabbit polyclonal antibody (STJ118557) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EML4 antibody (360-560) (STJ118557)

SKU:
STJ118557

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EML4 (360-560) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EML4
Gene ID: 27436
Uniprot ID: EMAL4_HUMAN
Immunogen Region: 360-560
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 360-560 of human EML4 (NP_061936.2).
Immunogen Sequence: GTFERGVGCLDFSKADSGVH LCVIDDSNEHMLTVWDWQKK AKGAEIKTTNEVVLAVEFHP TDANTIITCGKSHIFFWTWS GNSLTRKQGIFGKYEKPKFV QCLAFLGNGDVLTGDSGGVM LIWSKTTVEPTPGKGPKGVY QISKQIKAHDGSVFTLCQMR NGMLLTGGGKDRKIILWDHD LNPEREIEVPDQYGTIRAVA E
Post Translational Modifications Phosphorylated during mitosis. Phosphorylation at Ser-144 and Ser-146 promotes its dissociation from microtubules during mitosis which is required for efficient chromosome congression.
Function Essential for the formation and stability of microtubules (MTs). Required for the organization of the mitotic spindle and for the proper attachment of kinetochores to MTs. Promotes the recruitment of NUDC to the mitotic spindle for mitotic progression.
Protein Name Echinoderm Microtubule-Associated Protein-Like 4
Emap-4
Restrictedly Overexpressed Proliferation-Associated Protein
Ropp 120
Database Links Reactome: R-HSA-9648025
Reactome: R-HSA-9700645
Reactome: R-HSA-9725370
Reactome: R-HSA-9725371
Cellular Localisation Cytoplasm
Cytoskeleton
Spindle
Microtubule Organizing Center
Midbody
Localizes To Microtubules (Mts) During Interphase With A Significantly Reduced Affinity For Mts During Mitosis
Alternative Antibody Names Anti-Echinoderm Microtubule-Associated Protein-Like 4 antibody
Anti-Emap-4 antibody
Anti-Restrictedly Overexpressed Proliferation-Associated Protein antibody
Anti-Ropp 120 antibody
Anti-EML4 antibody
Anti-C2orf2 antibody
Anti-EMAPL4 antibody

Information sourced from Uniprot.org

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