• Immunofluorescence analysis of PC-12 cells using ENO3 antibody (STJ118799) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using ENO3 antibody (STJ118799) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using ENO3 antibody (STJ118799) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using ENO3 Rabbit polyclonal antibody (STJ118799) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ENO3 antibody (1-190) (STJ118799)

SKU:
STJ118799

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ENO3 (1-190) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ENO3
Gene ID: 2027
Uniprot ID: ENOB_HUMAN
Immunogen Region: 1-190
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human ENO3 (NP_001967.3).
Immunogen Sequence: MAMQKIFAREILDSRGNPTV EVDLHTAKGRFRAAVPSGAS TGIYEALELRDGDKGRYLGK GVLKAVENINNTLGPALLQK KLSVVDQEKVDKFMIELDGT ENKSKFGANAILGVSLAVCK AGAAEKGVPLYRHIADLAGN PDLILPVPAFNVINGGSHAG NKLAMQEFMILPVGASSFKE AMRIGAEVYH
Tissue Specificity The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Function Glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. Appears to have a function in striated muscle development and regeneration.
Protein Name Beta-Enolase
2-Phospho-D-Glycerate Hydro-Lyase
Enolase 3
Muscle-Specific Enolase
Mse
Skeletal Muscle Enolase
Database Links Reactome: R-HSA-70171
Reactome: R-HSA-70263
Cellular Localisation Cytoplasm
Localized To The Z Line
Some Colocalization With Ckm At M-Band
Alternative Antibody Names Anti-Beta-Enolase antibody
Anti-2-Phospho-D-Glycerate Hydro-Lyase antibody
Anti-Enolase 3 antibody
Anti-Muscle-Specific Enolase antibody
Anti-Mse antibody
Anti-Skeletal Muscle Enolase antibody
Anti-ENO3 antibody

Information sourced from Uniprot.org

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