Anti-Endoribonuclease Dicer antibody (1388-1405 aa) (STJA0040691)
SPECIFICATIONS
ClonalityPolyclonal
HostChicken
ConjugationUnconjugated
IsotypeIgY
ImmunogenA synthetic peptide from mouse Dicer protein (1388-1405 aa) conjugated to KLH.
General Information
| Short Description | Chicken polyclonal anti-Endoribonuclease Dicer (1388-1405 aa) for use in ICC, IHC-F and WB in Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ICC/IHC-F/WB |
| Host | Chicken |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgY |
| Conjugation | Unconjugated |
| Purification | Affinity-purified |
| Dilution Range | WB 1:1000-1:3000IHC 1:200-1:500 |
| Formulation | PBS with 0.02% sodium azide. |
| Storage Instruction | Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at |
Target Information
| Gene Symbol | Dicer1Uniprot ID=DICER_MOUSE" |
| Immunogen | A synthetic peptide from mouse Dicer protein (1388-1405 aa) conjugated to KLH. |
| Immunogen Region | 1388-1405 aa |
| Immunogen Sequence | Mouse |
| Specificity | Specificity confirmed by IHC and WB in mouse brain. mouse; rat;. |
Additional Info
| Tissue Specificity | Isoform 1 is expressed in a wide variety of tissues. Isoform 2 is specifically expressed in oocytes during their growth (at protein level). |
| Function | Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes. Isoform 2: More active than isoform 1 to process long double-stranded RNA into siRNAs. Responsible for the accumulation of endogenous siRNAs observed in mouse oocytes compared to somatic cells and it regulates meiotic spindle organization in female germline. |
| Protein Name | Endoribonuclease DicerDouble-Strand-Specific Ribonuclease Mdcr-1 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Endoribonuclease Dicer antibodyAnti-Double-Strand-Specific Ribonuclease Mdcr-1 antibodyAnti-Dicer1 antibodyAnti-Dicer antibodyAnti-Mdcr antibody |
Information sourced from Uniprot.org