Immunocytochemistry Antibodies | St John's Laboratory

Chromatin immunoprecipitation analysis of extracts of K-562 cells, using Ikaros antibody (STJ24155) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-IKZF1 antibody (1-270) (STJ24155)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

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CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g Ikaros rabbit monoclonal antibody (STJ11101900) , along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of Ikaros in representative gene loci (MICA) , as shown in figure.

Anti-IKZF1 antibody (250-350) [S0MR] (STJ11101900)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA/ChIP/CUT&Tag

Reactivity :

Human/Mouse

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Immunofluorescence analysis of MCF-7 cells using IKZF3 antibody (STJ29426). Blue: DAPI for nuclear staining.

Anti-IKZF3 antibody (280-450) (STJ29426)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of lysates from Raji cells, using IKZF3 Rabbit mAb (STJ11103626) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-IKZF3 antibody (400-509) [S6MR] (STJ11103626)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Confocal imaging of 293F cells transfected with IL-2RB using IL-2RB Rabbit mAb (STJ11107355, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-IL-2RB antibody (27-235 aa) [SRM] (STJ11107355)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse

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Anti-IL-33R antibody (27-332 aa) [SRM] (STJ11107190)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse

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Confocal imaging of HeLa cells using IL10 Rabbit monoclonal antibody (STJ11102745, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-IL10 antibody (1-100) [S5MR] (STJ11102745)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

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Immunofluorescence analysis of L929 cells using IL10RA Rabbit polyclonal antibody (STJ24158) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IL10RA antibody (265-578) (STJ24158)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of L929 cells using IL10RB Rabbit polyclonal antibody (STJ118126) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IL10RB antibody (20-220) (STJ118126)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of L929 cells using IL11 antibody (STJ29870) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-IL11 antibody (22-199) (STJ29870)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-IL12A antibody (23-219 aa) (STJ11107225)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

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Immunofluorescence analysis of U-2 OS cells using IL13RA2 Rabbit polyclonal antibody (STJ11103023) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IL13RA2 antibody (50-150) (STJ11103023)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of PC-12 cells using CD215/IL-15R alpha Rabbit polyclonal antibody (STJ11103511) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-IL15RA antibody (167-267) (STJ11103511)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various lysates, using IL17A antibody (STJ114328) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-IL17A antibody (1-100) (STJ114328)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of A549, using IL17C rabbit polyclonal antibody (STJ112601) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-IL17C antibody (19-197) (STJ112601)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot with Anti-IL17RA Antibody (STJ501422) with peptide at 1:750 dilution in buffer. 12% gel.

Anti-IL17RA antibody (110-150) (STJ501422)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human/Mouse/Rat

$577.13

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Anti-IL17RA antibody (110-150) {Biotin} (STJ501423)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-IL17RA antibody (110-150) {FITC} (STJ501424)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-IL18 antibody (1-193 aa) [SRM] (STJ11106987)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of A549 cells using IL18BP antibody (STJ28528). Blue: DAPI for nuclear staining.

Anti-IL18BP antibody (31-194) (STJ28528)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

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Western blot analysis of extracts of NCI-H460 cells, using IL18R1/IL-18 R alpha antibody (STJ24172) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IL18R1 antibody (19-320) (STJ24172)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of THP-1 cells using IL1 Beta Rabbit polyclonal antibody (STJ113778) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IL1B antibody (1-269) (STJ113778)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

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Western blot analysis of various lysates using IL1 Beta Rabbit pAb (STJ24174) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-IL1B antibody (1-269) (STJ24174)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of extracts of THP-1 cells, using IL1 Beta antibody (STJ11101739) at 1:1000 dilution. THP-1 cells were treated by LPS (1 Mu g/mL) at 37 °C for 8 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-IL1B antibody (117-269) [S9MR] (STJ11101739)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

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Western blot analysis of recombinant mouse IL-1 beta protein, using IL1 Beta Rabbit monoclonal antibody (STJ11104780) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10ng/5ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-IL1Beta antibody (118-269) [S4780RM] (STJ11104780)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse

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Western blot analysis of various lysates using IL36R Rabbit polyclonal antibody (STJ112130) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-IL1RL2 antibody (1-116) (STJ112130)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

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Confocal imaging of mouse tail ear using IL1RA Rabbit monoclonal antibody (STJ11101277, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-IL1RN antibody (100-177) [S7MR] (STJ11101277)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of extracts of A-431 cells, using IL1RA antibody (STJ24179) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IL1RN antibody (24-177) (STJ24179)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of NIH/3T3 cells using IL1RN Rabbit polyclonal antibody (STJ11104175) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-IL1RN antibody (27-178) (STJ11104175)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

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Western blot analysis of extracts of various cell lines, using (STJ29315) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-IL21 antibody (25-162) (STJ29315)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of extracts of normal 293T cells and 293T transfected with IL22 using IL22 Rabbit mAb (STJ11107003) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 45s.

Anti-IL22 antibody (34-179 aa) [SRM] (STJ11107003)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of HL-60, using IL22 antibody (STJ27972) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-IL22 antibody (34-179) (STJ27972)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of L929 cells using IL23R Rabbit polyclonal antibody (STJ24181) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-IL23R antibody (510-629) (STJ24181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with 17E/IL25 using IL25 Rabbit pAb (STJ110551) at 1:2000 dilution. <br/> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/> Lysates/ proteins: 25 Mu g per lane. <br/> Blocking buffer: 3 % nonfat dry milk in TBST. <br/> Detection: ECL Basic Kit <br/> Exposure time: 90s.

Anti-IL25 antibody (33-177) (STJ110551)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of extracts of various cell lines, using CD25 antibody (STJ24183) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-IL2RA antibody (153-167) (STJ24183)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/FC/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-IL33 antibody (109-270 aa) [SRM] (STJ11107280)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

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Western blot analysis of extracts of various cell lines, using IL36G antibody (STJ112204) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-IL36G antibody (1-193) (STJ112204)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

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Immunofluorescence analysis of paraffin-embedded Human breast cancer tissue using IL4 Rabbit polyclonal antibody (STJ27616) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-IL4 antibody (25-153) (STJ27616)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

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Western blot analysis of various lysates using IL5 Rabbit pAb (STJ110003) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit Exposure time: 15s.

Anti-IL5 antibody (20-134) (STJ110003)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of recombinant Human IL-5 Protein, using Human IL-5 Rabbit polyclonal antibody (STJ11104218) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10ng/5ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 8s.

Anti-IL5 antibody (20-134) (STJ11104218)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

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Immunofluorescence analysis of U-87MG cells using IL6 Rabbit monoclonal antibody (STJ11104247) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-IL6 antibody (25-211) [S4247RM] (STJ11104247)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human/Rat

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Immunofluorescence analysis of NIH/3T3 cells using IL6 Rabbit polyclonal antibody (STJ110936) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IL6 antibody (30-212) (STJ110936)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of U2OS cells using IL6ST Rabbit polyclonal antibody (STJ11100009) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IL6ST antibody (751-918) (STJ11100009)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of extracts of various cell lines, using CD127/IL7R rabbit monoclonal antibody (STJ11101386) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IL7R antibody (300-400) [S6MR] (STJ11101386)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from THP-1 cells using IL8 Rabbit mAb (STJ11107026) at 1:1000 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37C for overnight and LPS (1 Mu g/ml) at 37C for 6 hours. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.

Anti-IL8 antibody (28-99 aa) [SRM] (STJ11107026)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g ILF3 antibody (STJ24197). Western blot was performed from the immunoprecipitate using ILF3 antibody (STJ24197) at a dilution of 1:1000.

Anti-ILF3 antibody (1-270) (STJ24197)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Confocal imaging of 293F cells transfected with ILT4 using ILT4 Rabbit mAb (STJ11107370, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-ILT4 antibody (22-461 aa) [SRM] (STJ11107370)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

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Immunofluorescence analysis of NIH/3T3 cells using IMMP2L Rabbit polyclonal antibody (STJ11105236) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IMMP2L antibody (38-175) (STJ11105236)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

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