Immunocytochemistry Antibodies | St John's Laboratory

Anti-Phospho-Beta-Tubulin-Ser-172 antibody (STJA0041423)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£542.50

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Western blot analysis of lysates from HT-29 cells, using Phospho-BRCA1-S1423 Rabbit pAb (STJ22043) at 1:2000 dilution. HT-29 cells were treated by Anisomycin (5 Mu g/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-BRCA1-S1423 antibody (STJ22043)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Phospho-BRCA1-S988 Rabbit pAb (STJ22045) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.

Anti-Phospho-BRCA1-S988 antibody (STJ22045)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human

£126.50 - £618.50

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Phospho-BTK Tyr551 antibody western blot Ramos lysates STJA0036067

Anti-Phospho-BTK-Tyr551 antibody [R07-9C1] (STJA0036067)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-CAV1-Tyr-14 antibody [M283] (STJA0005314)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£390.50

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Western blot analysis caveolin-1 antibody in human A431 cells with pervanadate stimulation STJA0041116

Anti-Phospho-Caveolin-1-Tyr-14 antibody [M283] (STJA0041116)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£579.50

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Western blot analysis of various lysates using Phospho-Cyclin E1-T395 Rabbit pAb (STJ22052). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-CCNE1-T395 antibody (STJ22052)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Phospho-CDC6 Ser54 antibody western blot Raji lysates STJA0036181

Anti-Phospho-CDC6-Ser54 antibody [R04-6V2] (STJA0036181)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-CDH2-Tyr-820 antibody (STJA0005287)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

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Anti-Phospho-CDH2-Tyr-860 antibody (STJA0005290)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

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Western blot analysis of lysates from HeLa cells, using Phospho-CDK1-T161 Rabbit pAb (STJ22061) at 1:400 dilution. HeLa cells were treated by EGF. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-CDK1-T161 antibody (STJ22061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-CDK1-Y15 Rabbit pAb (STJ23048). HeLa cells were treated by nocodazole (50 ng/mL) at 37 °C for 20 hours or Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-CDK1-Y15 antibody (STJ22059)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CDK4-T172 Rabbit pAb (STJ23055). NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-CDK4-T172 antibody (STJ29359)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from 293 cells using Phospho-CDK6-Y13 Rabbit pAb (STJ22064). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-CDK6-Y13 antibody (STJ22064)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Western blot analysis of lysates from 293 cells using Phospho-CDK6-Y24 Rabbit pAb (STJ22063). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-CDK6-Y24 antibody (STJ22063)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse

£126.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CFL1-S3 Rabbit pAb (STJ26176). NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-CFL1-S3 antibody (STJ22068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Phospho-CFL1-Ser-3 antibody (STJA0005346)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£365.50

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Western blot analysis of various lysates using Phospho-c-Jun-T91 Rabbit pAb (STJ11103749) at 1:1000 dilution. Both 293T cells and NIH/3T3 cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. Both 293T cells and NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-cJun-T91 antibody (STJ11103749)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot cofilin phosphorylation in Jurkat cells with phosphatase treatment STJA0041140

Anti-Phospho-Cofilin 1-Ser-3 antibody (STJA0041140)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£542.50

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Anti-Phospho-CORO1B-Ser-2 antibody (STJA0005349)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

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Western blot anti-Coronin-1B antibody in A431 cells with phosphatase treatment comparison STJA0041154

Anti-Phospho-Coronin-1B-Ser-2 antibody (STJA0041154)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£542.50

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Western blot anti-CREB antibody phosphatase treatment in A431 cells STJA0041123

Anti-Phospho-CREB-Ser-133 antibody [M415] (STJA0041123)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£474.50

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Western blot anti-CREB antibody phosphatase treatment in A431 cells STJA0041165

Anti-Phospho-CREB-Ser-271 antibody (STJA0041165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£542.50

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Western blot analysis of lysates from HeLa cells, using Phospho-CREB1-S133 Rabbit pAb (STJ227724). HeLa cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-CREB1-S133 antibody (STJ22076)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Phospho-CREB1-Ser-133 antibody [M415] (STJA0005351)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£319.50

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Anti-Phospho-CREB1-Ser-271 antibody (STJA0005352)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£365.50

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Phospho-CrkII antibody western blot K562 lysates STJA0036028

Anti-Phospho-CRK-Tyr221 antibody [R04-6L8] (STJA0036028)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Western blot CRMP2 detection in mouse brain with and without phosphatase treatment STJA0041150

Anti-Phospho-CRMP2-Ser-522 antibody (STJA0041150)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£542.50

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western blot CRMP2 detection in rat PC12 cells with calyculin A treatment STJA0041152

Anti-Phospho-CRMP2-Thr-555 antibody (STJA0041152)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£542.50

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Western blot analysis of lysates from C6 cells, using Phospho-Beta-Catenin-S675 Rabbit pAb (STJ117893) at 1:1000 dilution. C6 cell lysates were treated by CIP at 37°C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-CTNNB1-S675 antibody (STJ117893)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Phospho-CTNNB1-Tyr-142 antibody (STJA0005304)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

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Anti-Phospho-CTNNB1-Tyr-489 antibody (STJA0005306)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

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Anti-Phospho-CTNNB1-Tyr-654 antibody (STJA0005307)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£365.50

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Anti-Phospho-CTNNB1-Tyr-86 antibody (STJA0005303)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

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Anti-Phospho-CTNND1-Tyr-228 antibody [M356] (STJA0005311)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£390.50

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Anti-Phospho-CXCR4-S324/S325 antibody (C-Term) (STJA0005364)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IHC

Reactivity :

Chicken/Human/Mouse/Rat

£365.50

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Western blot CXCR4 phosphorylation in Jurkat cells with phosphatase treatment comparison STJA0041168

Anti-Phospho-CXCR4-S324/S325 antibody (C-Term) (STJA0041168)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC/IHC

Reactivity :

Chicken/Human/Mouse/Rat

£542.50

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Phospho-Cyclin E1 antibody western blot JAR cells STJA0036119

Anti-Phospho-Cyclin E1-Thr77 antibody [R02-6U7] (STJA0036119)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Phospho-Cytokeratin 8 antibody western blot K562 HeLa STJA0034229

Anti-Phospho-Cytokeratin 8-Ser23 antibody [R03-1B3] (STJA0034229)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Phospho-delta 1 Catenin antibody western blot MCF-7 U251 C2C12 STJA0035059

Anti-Phospho-delta 1 Catenin-Thr916 antibody [R07-5A6] (STJA0035059)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse

£133.50 - £281.50

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Phospho-delta 1 Catenin Tyr228 antibody immunocytochemistry SKOV-3 STJA0033919

Anti-Phospho-delta 1 Catenin-Tyr228 antibody [R08-5A8] (STJA0033919)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Rat/Hamster

£133.50 - £281.50

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Anti-Phospho-Delta 1-Catenin-Tyr-228 antibody [M356] (STJA0041119)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£579.50

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Phospho-DNA PKcs Ser2056 antibody western blot Jurkat STJA0035811

Anti-Phospho-DNA PKcs-Ser2056 antibody [R02-3K5] (STJA0035811)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Western blot dynamin phosphorylated at Ser774 in rat hippocampal lysate STJA0003622

Anti-Phospho-DNM1-Ser774 antibody (STJA0003622)

Clonality :

Polyclonal

Host :

Sheep

Applications :

WB/ICC

Reactivity :

Mouse/Rat/Bovine/Canine/Chicken/Human/Non-Human Primates

£488.50

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Immunofluorescence analysis of MCF-7 cells using Phospho-DNMT1-S714 Rabbit pAb (STJ22088). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Phospho-DNMT1-pS714 antibody (STJ22088)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from Jurkat cells using Phospho-DOK2-Y299 Rabbit pAb (STJ11105773). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-DOK2-Y299 antibody (STJ11105773)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human

£126.50 - £618.50

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Anti-Phospho-DPYSL2-Ser-522 antibody (STJA0005359)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

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Anti-Phospho-DPYSL2-Thr-555 antibody (STJA0005360)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

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