• Western blot analysis of extracts of various cell lines, using Phospho-c-Jun-T91 antibody (STJ11103749) at 1:1000 dilution. Both 293T cells and NIH/3T3 cells were treated by Anisomycin (25 ug/ml) at 37 °C for 30 minutes after serum-starvation overnight. Both 293T cells and NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of U2OS cells using Phospho-c-Jun-T91 Rabbit polyclonal antibody (STJ11103749) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat lung using Phospho-c-Jun-T91 rabbit polyclonal antibody (STJ11103749) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Phospho-c-Jun-T91 in paraffin-embedded mouse kidney using Phospho-c-Jun-T91 Rabbit polyclonal antibody (STJ11103749) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human liver cancer using Phospho-c-Jun-T91 rabbit polyclonal antibody (STJ11103749) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Phospho-c-Jun-T91 in paraffin-embedded human liver cancer using Phospho-c-Jun-T91 Rabbit polyclonal antibody (STJ11103749) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-c-Jun-T91 rabbit polyclonal antibody (STJ11103749) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Phospho-c-Jun-T91 in paraffin-embedded rat lung using Phospho-c-Jun-T91 Rabbit polyclonal antibody (STJ11103749) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U2OS cells using Phospho-c-Jun-T91 rabbit polyclonal antibody (STJ11103749) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using Phospho-c-Jun-T91 Rabbit polyclonal antibody (STJ11103749) at 1:1000 dilution. Both 293T cells and NIH/3T3 cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. Both 293T cells and NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-cJun-T91 antibody (STJ11103749)

SKU:
STJ11103749

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-cJun-T91 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: JUN
Gene ID: 3725
Uniprot ID: JUN_HUMAN
Immunogen: A synthetic phosphorylated peptide around T91 of human JUN (NP_002219.1).
Immunogen Sequence: TTTPT
Tissue Specificity Expressed in the developing and adult prostate and prostate cancer cells.
Post Translational Modifications Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7. Phosphorylated by CaMK4 and PRKDC.phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243.this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B.phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity. Acetylated at Lys-271 by EP300.
Function Transcription factor that recognizes and binds to the AP-1 consensus motif 5'-TGAGCTCA-3'. Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to the AP-1 consensus sequence 5'-TGAGCTCA-3' and enhancing its transcriptional activity. Together with FOSB, plays a role in activation-induced cell death of T cells by binding to the AP-1 promoter site of FASLG/CD95L, and inducing its transcription in response to activation of the TCR/CD3 signaling pathway. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells. Binds to the USP28 promoter in colorectal cancer (CRC) cells. (Microbial infection) Upon Epstein-Barr virus (EBV) infection, binds to viral BZLF1 Z promoter and activates viral BZLF1 expression.
Protein Name Transcription Factor Jun
Activator Protein 1
Ap1
Proto-Oncogene C-Jun
Transcription Factor Ap-1 Subunit Jun
V-Jun Avian Sarcoma Virus 17 Oncogene Homolog
P39
Database Links Reactome: R-HSA-1912408
Reactome: R-HSA-2559580
Reactome: R-HSA-2559582
Reactome: R-HSA-2871796
Reactome: R-HSA-450341
Reactome: R-HSA-5617472
Reactome: R-HSA-5687128
Reactome: R-HSA-6796648
Reactome: R-HSA-8862803
Reactome: R-HSA-8943724
Reactome: R-HSA-9018519
Reactome: R-HSA-9673324
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor Jun antibody
Anti-Activator Protein 1 antibody
Anti-Ap1 antibody
Anti-Proto-Oncogene C-Jun antibody
Anti-Transcription Factor Ap-1 Subunit Jun antibody
Anti-V-Jun Avian Sarcoma Virus 17 Oncogene Homolog antibody
Anti-P39 antibody
Anti-JUN antibody

Information sourced from Uniprot.org

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