| Host: |
Rabbit |
| Applications: |
WB/ICC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-CRMP2-Ser-522 is suitable for use in Western Blot and Immunocytochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:1000IHCICC 1:100IP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DPYSL2 |
| Gene ID: |
1808 |
| Uniprot ID: |
DPYL2_HUMAN |
| Specificity: |
The antibody detects a 70 kDa* protein corresponding to the molecular mass of CRMP2 on SDS-PAGE immunoblots of human mouse brain, and this band is removed after lambda phosphatase treatment. In addition, this reactivity can be specifically blocked us |
| Immunogen: |
Phospho-CRMP2 (Ser-522) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-522 in human CRMP2. This sequence is conserved in rat and mouse CRMP2, and has homology to the conserved site in CRMP1 and CRMP4, but |
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | 3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509. Phosphorylation at Thr-514 by GSK3B abolishes tubulin-binding leading to destabilization of microtubule assembly in axons and neurodegeneration. Phosphorylation by DYRK2 at Ser-522 is required for subsequent phosphorylation by GSK3B. |
| Function | Plays a role in neuronal development and polarity, as well as in axon growth and guidance, neuronal growth cone collapse and cell migration. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. May play a role in endocytosis. |
| Protein Name | Dihydropyrimidinase-Related Protein 2Drp-2Collapsin Response Mediator Protein 2Crmp-2N2a3Unc-33-Like Phosphoprotein 2Ulip-2 |
| Database Links | Reactome: R-HSA-399956Reactome: R-HSA-437239 |
| Cellular Localisation | CytoplasmCytosolCytoskeletonMembraneTightly But Non-Covalently Associated With Membranes |
| Alternative Antibody Names | Anti-Dihydropyrimidinase-Related Protein 2 antibodyAnti-Drp-2 antibodyAnti-Collapsin Response Mediator Protein 2 antibodyAnti-Crmp-2 antibodyAnti-N2a3 antibodyAnti-Unc-33-Like Phosphoprotein 2 antibodyAnti-Ulip-2 antibodyAnti-DPYSL2 antibodyAnti-CRMP2 antibodyAnti-ULIP2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
