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Anti-Phospho-CCNE1-T395 antibody (STJ22052)

Western blot analysis of extracts of various cell lines, using Phospho-Cyclin E1-T395 antibody (STJ22052). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
Immunofluorescence analysis of U2OS cells using Phospho-Cyclin E1-T395 Rabbit polyclonal antibody (STJ22052) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using Phospho-Cyclin E1-T395 rabbit polyclonal antibody (STJ22052) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Phospho-Cyclin E1-T395 Rabbit polyclonal antibody (STJ22052) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Phospho-Cyclin E1-T395 rabbit polyclonal antibody (STJ22052) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using Phospho-Cyclin E1-T395 Rabbit polyclonal antibody (STJ22052) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using Phospho-Cyclin E1-T395 rabbit polyclonal antibody (STJ22052) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using Phospho-Cyclin E1-T395 antibody (STJ22052). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA.

Anti-Phospho-CCNE1-T395 antibody (STJ22052)

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STJ22052

£212.50 - £501.50

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Phospho-CCNE1-T395 is suitable for use in Western Blot and Immunofluorescence research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:2000 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	CCNE1
Gene ID:	898
Uniprot ID:	CCNE1_HUMAN
Immunogen:	A synthetic phosphorylated peptide around T395 of human Cyclin E1 (NP_001229.1).
Immunogen Sequence:	LLTPP

Tissue Specificity	Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
Post Translational Modifications	Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7. Ubiquitinated by UHRF2.appears to occur independently of phosphorylation.
Function	Essential for the control of the cell cycle at the G1/S (start) transition.
Protein Name	G1/S-Specific Cyclin-E1
Database Links	Reactome: R-HSA-1538133 Reactome: R-HSA-187577 Reactome: R-HSA-2559586 Reactome: R-HSA-390471 Reactome: R-HSA-6804116 Reactome: R-HSA-69017 Reactome: R-HSA-69200 Reactome: R-HSA-69202 Reactome: R-HSA-69205 Reactome: R-HSA-69231 Reactome: R-HSA-69563 Reactome: R-HSA-8849470 Reactome: R-HSA-9661069 Reactome: R-HSA-9706019
Cellular Localisation	Nucleus
Alternative Antibody Names	Anti-G1/S-Specific Cyclin-E1 antibody Anti-CCNE1 antibody Anti-CCNE antibody

Information sourced from Uniprot.org

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