Anti-Phospho-Coronin-1B-Ser-2 antibody (STJA0041154)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenCoronin-1B (Ser-2) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding serine 2 in human coronin-1B. This sequence has high homology to the conserved site in rat and mouse coronin-1B and in coronin-1A. However, this
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Coronin-1B-Ser-2 for use in ELISA, WB and ICC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB/ICC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Antigen Affinity Purified |
| Dilution Range | WB 1:1000ICC 1:100 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Storage at-20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20°C. |
Target Information
| Gene Symbol | CORO1B |
| Gene ID | 57175 |
| Uniprot ID | COR1B_HUMAN |
| Immunogen | Coronin-1B (Ser-2) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding serine 2 in human coronin-1B. This sequence has high homology to the conserved site in rat and mouse coronin-1B and in coronin-1A. However, this |
| Immunogen Sequence | Human |
| Specificity | The antibody detects a 60 kDa* band corresponding to Coronin-1B on SDS-PAGE immunoblots of human A431 or HeLa cells treated with Calyculin A. This band is not observed in control cells and the band observed in Calyculin A-treated cells can be removed |
Additional Info
| Post Translational Modifications | Phosphorylation by PKC on Ser-2 regulates the interaction with the Arp2/3 complex and cell motility in fibroblasts. Phosphorylation does not seem to affect subcellular location. |
| Function | Regulates leading edge dynamics and cell motility in fibroblasts. May be involved in cytokinesis and signal transduction. |
| Protein Name | Coronin-1bCoronin-2 |
| Database Links | |
| Cellular Localisation | CytoplasmCytoskeletonStress FiberLocalized To The Leading Edge In FibroblastsAs Well As Weakly Along Actin Stress Fibers |
| Alternative Antibody Names | Anti-Coronin-1b antibodyAnti-Coronin-2 antibodyAnti-CORO1B antibody |
Information sourced from Uniprot.org