ELISA Antibodies | St John's Laboratory

STJ71082 (0. 1µg/ml) staining of Daudi cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-AS160/TBC1D4 antibody (Internal) (STJ71082)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Dog

$421.88

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Western blot analysis of lysates from RT4 cells using ASAH1 rabbit polyclonal antibody (STJ115883) at 1:700 dilution. Secondary antibody:HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:60s.

Anti-ASAH1 antibody (140-389) (STJ115883)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using ASAH1 antibody (STJ28610) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ASAH1 antibody (140-389) (STJ28610)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using ASAH2 Rabbit pAb (STJ110292) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-ASAH2 antibody (531-780) (STJ110292)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using ASAH2 antibody (STJ114470) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ASAH2 antibody (80-340) (STJ114470)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using ASAP2 Rabbit polyclonal antibody (STJ119651) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ASAP2 antibody (1-120) (STJ119651)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$151.88 - $708.08

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ASAP2 antibody (640-720 aa) (STJ190149)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Anti-ASB10 antibody (STJA0004637)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$189.68 - $560.93

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Western blot analysis of various lysates, using ASB13 antibody (STJ118342) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ASB13 antibody (1-278) (STJ118342)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using ASB17 Rabbit polyclonal antibody (STJ119846) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ASB17 antibody (30-130) (STJ119846)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from Rat testis, using ASB17 Rabbit polyclonal antibody (STJ11103238) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ASB17 antibody (78-177) (STJ11103238)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$151.88 - $708.08

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Western blot analysis of lysates from 293T cells, using ASB2 Rabbit polyclonal antibody (STJ119909) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ASB2 antibody (448-587) (STJ119909)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using ASB3 Rabbit pAb (STJ11106526) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-ASB3 antibody (1-145 aa) (STJ11106526)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunohistochemistry analysis of ASB4 in paraffin-embedded mouse kidney using ASB4 Rabbit polyclonal antibody (STJ119327) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ASB4 antibody (1-300) (STJ119327)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Anti-ASB6 antibody (STJA0004830)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse

$189.68 - $560.93

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Anti-ASB8 antibody (STJA0004775)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse

$189.68 - $560.93

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Anti-ASC antibody [PT0530R] (STJA0039308)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Mouse/Rat

$0.00

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Anti-ASC antibody [PT0657R] (STJA0040054)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

$0.00

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Western blot analysis of various lysates, using ASC/TMS1 antibody (STJ11104876) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ASC/TMS1 antibody (1-100 aa) (STJ11104876)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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STJ70108 (0. 2µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ASC/TMS1 antibody (C-Term) (STJ70108)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

$421.88

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Biotinylated STJ70108 (3µg/ml) staining of U937 lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-ASC/TMS1, Biotinylated antibody (C-Term) {Biotin} (STJ73188)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

$421.88

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Anti-ASC/TMS1/PYCARD nanobody [SAA1025] (STJN000312)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

$510.98 - $2,203.88

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Anti-ASC/TMS1/PYCARD nanobody [SAA1187] (STJN000311)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA/IF/IP

Reactivity :

Human

$510.98 - $2,203.88

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Western blot analysis of lysates from rat pancreas, using ASCC1 Rabbit polyclonal antibody (STJ118277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ASCC1 antibody (1-180) (STJ118277)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using ASCC2 Rabbit polyclonal antibody (STJ115734) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ASCC2 antibody (488-757) (STJ115734)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates, using ASCC3 Rabbit pAb (STJ110267) at 1:800 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 50s.

Anti-ASCC3 antibody (1-111) (STJ110267)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Immunohistochemistry analysis of ASCL1 antibody in paraffin-embedded human brain tissue.

Anti-ASCL1 antibody (109-158 aa) (STJ91732)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various lysates using AscL1 Rabbit polyclonal antibody (STJ118012) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-AscL1 antibody (150-236) (STJ118012)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

$151.88 - $708.08

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Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:200

Anti-ASCL1 antibody (190-236 aa) (STJ98708)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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STJ73536 (1µg/ml) staining of Mouse (A) and Rat (B) Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Anti-ASCL1 antibody (79-91) (STJ73536)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Human/Cow/Mouse/Rat/Dog

$421.88

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Anti-ASCL1 antibody [SAA1643] (STJA0020071)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

$461.03 - $1,954.13

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Anti-Ascl1a antibody (Internal) (STJ71255)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA

Reactivity :

Zebrafish

$261.23

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Anti-ASCL2 antibody (1-80 aa) (STJ94020)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Ascl2 antibody (185-263 aa) (STJ11106594)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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STJ72471 (0. 1µg/ml) staining of Mouse Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Ascl3-Mouse antibody (Internal) (STJ72471)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Cow

$421.88

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Immunohistochemistry analysis of paraffin-embedded human breast cancer using ASCL4 antibody (STJ116649) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ASCL4 antibody (1-70) (STJ116649)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-ASF1A antibody (100-204) (STJ116719)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of various cells using Asf1a Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-ASF1A antibody (121-170 aa) (STJ91733)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot with Anti-ASF1A Antibody (STJ500171) of rat muscle membranes at 1:500 dilution in buffer.

Anti-ASF1A antibody (60-120) (STJ500171)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

DB/ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$577.13

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Anti-ASF1A antibody (60-120) {Biotin} (STJ500172)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

DB/ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-ASF1A antibody (60-120) {FITC} (STJ500173)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

DB/ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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STJ71364 (1µg/ml) staining of Jurkat cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ASF1A antibody (C-Term) (STJ71364)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog

$421.88

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STJ71180 (0. 01µg/ml) staining of Jurkat cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ASF1A/HSPC146 antibody (Internal) (STJ71180)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog

$421.88

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Western blot analysis of lysates from K562 cells, using ASF1B Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-ASF1B antibody (101-150 aa) (STJ91734)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using ASF1B antibody (STJ28594) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ASF1B antibody (63-202) (STJ28594)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Anti-ASF1B IP antibody [SAA0242] (STJA0018757)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IP

Reactivity :

Human

$510.98 - $2,203.88

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Anti-ASFV 9GL/pB119L/P14 antibody [1A021] (STJA0019897)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

ASFV

$309.83 - $443.48

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Anti-ASFV A238L antibody (Met1-Val226) [1A449] (STJA0022396)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

ASFV

$309.83 - $443.48

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