• Western blot analysis of extracts of SKOV3 cells, using ARL13B antibody (STJ27176) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of C6 cells using ARL13B antibody (STJ27176) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using ARL13B antibody (STJ27176) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using ARL13B antibody (STJ27176) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ARL13B antibody (1-240) (STJ27176)

SKU:
STJ27176

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARL13B (1-240) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARL13B
Gene ID: 200894
Uniprot ID: AR13B_HUMAN
Immunogen Region: 1-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ARL13B (NP_878899.1).
Immunogen Sequence: MFSLMASCCGWFKRWREPVR KVTLLMVGLDNAGKTATAKG IQGEYPEDVAPTVGFSKINL RQGKFEVTIFDLGGGIRIRG IWKNYYAESYGVIFVVDSSD EERMEETKEAMSEMLRHPRI SGKPILVLANKQDKEGALGE ADVIECLSLEKLVNEHKCLC QIEPCSAISGYGKKIDKSIK KGLYWLLHVIARDFDALNER IQKETTEQRALEEQEKQERA ERVRKLREERKQNEQEQAE
Tissue Specificity Expressed in the developing brain.
Post Translational Modifications Sumoylation is required for PKD2 entry into cilium.
Function Cilium-specific protein required to control the microtubule-based, ciliary axoneme structure. May act by maintaining the association between IFT subcomplexes A and B. Binds GTP but is not able to hydrolyze it.the GTPase activity remains unclear. Required to pattern the neural tube. Involved in cerebral cortex development: required for the initial formation of a polarized radial glial scaffold, the first step in the construction of the cerebral cortex, by regulating ciliary signaling. Regulates the migration and placement of postmitotic interneurons in the developing cerebral cortex. May regulate endocytic recycling traffic.however, additional evidence is required to confirm these data.
Protein Name Adp-Ribosylation Factor-Like Protein 13b
Adp-Ribosylation Factor-Like Protein 2-Like 1
Arl2-Like Protein 1
Database Links Reactome: R-HSA-5624958
Reactome: R-HSA-9013406
Reactome: R-HSA-9013409
Reactome: R-HSA-9613829
Reactome: R-HSA-9615710
Reactome: R-HSA-9646399
Cellular Localisation Cell Projection
Cilium Membrane
Lipid-Anchor
Cilium
Associates To The Cilium Membrane Via Palmitoylation
Localizes To Proximal Ciliary Membranes
To An Inversin-Like Subciliary Membrane Compartment
Excluding The Transition Zone
Alternative Antibody Names Anti-Adp-Ribosylation Factor-Like Protein 13b antibody
Anti-Adp-Ribosylation Factor-Like Protein 2-Like 1 antibody
Anti-Arl2-Like Protein 1 antibody
Anti-ARL13B antibody
Anti-ARL2L1 antibody

Information sourced from Uniprot.org

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