Anti-ARL3 antibody (1-182) (STJ118776)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ARL3 (1-182) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ARL3 |
| Gene ID | 403 |
| Uniprot ID | ARL3_HUMAN |
| Immunogen Region | 1-182 |
| Immunogen Sequence | MGLLSILRKLKSAPDQEVRI LLLGLDNAGKTTLLKQLASE DISHITPTQGFNIKSVQSQG FKLNVWDIGGQRKIRPYWKN YFENTDILIYVIDSADRKRF EETGQELAELLEEEKLSCVP VLIFANKQDLLTAAPASEIA EGLNLHTIRDRVWQIQSCSA LTGEGVQDGMNWVCKNVNAK KK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-182 of human ARL3 (NP_004302.1). |
Additional Info
| Tissue Specificity | Expressed in the retina. Strongly expressed in connecting cilium, the myoid region of the inner segments (IS) and in cone photoreceptors (at protein level). |
| Function | Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins to the cilium, including myristoylated NPHP3 and prenylated INPP5E. Targets NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium. Required for PKD1:PKD2 complex targeting from the trans-Golgi network to the cilium. |
| Protein Name | Adp-Ribosylation Factor-Like Protein 3 |
| Database Links | Reactome: R-HSA-5624138 |
| Cellular Localisation | Golgi Apparatus MembranePeripheral Membrane ProteinCytoplasmic SideCytoplasmCytoskeletonSpindleNucleusMicrotubule Organizing CenterCentrosomeCell ProjectionCiliumDetected Predominantly In The Photoreceptor Connecting CiliumPresent On The Mitotic SpindleCentrosome-Associated Throughout The Cell CycleNot Detected To Interphase Microtubules |
| Alternative Antibody Names | Anti-Adp-Ribosylation Factor-Like Protein 3 antibodyAnti-ARL3 antibodyAnti-ARFL3 antibody |
Information sourced from Uniprot.org