• Western blot analysis of extracts of various cell lines, using ARL3 antibody (STJ118776) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of C6 cells using ARL3 Polyclonal Antibody (STJ118776) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using ARL3 Polyclonal Antibody (STJ118776) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ARL3 antibody (1-182) (STJ118776)

SKU:
STJ118776

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARL3 (1-182) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARL3
Gene ID: 403
Uniprot ID: ARL3_HUMAN
Immunogen Region: 1-182
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-182 of human ARL3 (NP_004302.1).
Immunogen Sequence: MGLLSILRKLKSAPDQEVRI LLLGLDNAGKTTLLKQLASE DISHITPTQGFNIKSVQSQG FKLNVWDIGGQRKIRPYWKN YFENTDILIYVIDSADRKRF EETGQELAELLEEEKLSCVP VLIFANKQDLLTAAPASEIA EGLNLHTIRDRVWQIQSCSA LTGEGVQDGMNWVCKNVNAK KK
Tissue Specificity Expressed in the retina. Strongly expressed in connecting cilium, the myoid region of the inner segments (IS) and in cone photoreceptors (at protein level).
Function Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins to the cilium, including myristoylated NPHP3 and prenylated INPP5E. Targets NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium. Required for PKD1:PKD2 complex targeting from the trans-Golgi network to the cilium.
Protein Name Adp-Ribosylation Factor-Like Protein 3
Database Links Reactome: R-HSA-5624138
Cellular Localisation Golgi Apparatus Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasm
Cytoskeleton
Spindle
Nucleus
Microtubule Organizing Center
Centrosome
Cell Projection
Cilium
Detected Predominantly In The Photoreceptor Connecting Cilium
Present On The Mitotic Spindle
Centrosome-Associated Throughout The Cell Cycle
Not Detected To Interphase Microtubules
Alternative Antibody Names Anti-Adp-Ribosylation Factor-Like Protein 3 antibody
Anti-ARL3 antibody
Anti-ARFL3 antibody

Information sourced from Uniprot.org

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