ELISA Antibodies | St John's Laboratory

Western blot analysis of extracts of various cell lines, using CHCHD2 antibody (STJ119068) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CHCHD2 antibody (75-145) (STJ119068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from wild type (WT) and CHCHD3 knockout (KO) 293T cells, using [KO Validated] CHCHD3 Rabbit polyclonal antibody (STJ11100835) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CHCHD3 antibody (1-227) (STJ11100835)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates using CHCHD3 Rabbit pAb (STJ111317) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 40s.

Anti-CHCHD3 antibody (1-227) (STJ111317)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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STJ72149 (0. 3µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CHCHD3 antibody (151-164) (STJ72149)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Cow/Sheep

£312.50

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STJ72148 (1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CHCHD3 antibody (Internal) (STJ72148)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Cow/Sheep

£312.50

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Immunohistochemistry analysis of CHCHD4 in paraffin-embedded human liver cancer tissue using CHCHD4 Rabbit polyclonal antibody (STJ11105536) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-CHCHD4 antibody (1-105) (STJ11105536)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using CHCHD4 Rabbit polyclonal antibody (STJ115105) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CHCHD4 antibody (1-142) (STJ115105)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-CHCHD4 antibody [SRM14957] (STJ11109687)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of various lysates using CHCHD6 Rabbit polyclonal antibody (STJ114777) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CHCHD6 antibody (1-235) (STJ114777)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Immunofluorescence analysis of L929 cells using CHCHD7 Rabbit polyclonal antibody (STJ11100443) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CHCHD7 antibody (1-97) (STJ11100443)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded mouse colon using CHD1 Rabbit monoclonal antibody (STJ11105059) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CHD1 antibody (1510-1710 aa) [S5059RM] (STJ11105059)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Anti-CHD1 antibody (960-1040 aa) (STJ190179)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from wild type (WT) and CHD1 knockout (KO) 293T cells, using [KO Validated] CHD1 Rabbit pAb (STJ110193) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-CHD1 antibody (C-Term) (STJ110193)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-CHD1 antibody (Internal) (STJ71194)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA

Reactivity :

Human/Mouse/Rat/Dog

£193.50

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Western blot analysis of extracts from NIH-3T3, Jurkat, A549 cells, using CHD1L Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-CHD1L antibody (545-594 aa) (STJ96392)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using CHD2 antibody (STJ28168) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CHD2 antibody (1-223) (STJ28168)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using CHD2 antibody (STJ115439) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CHD2 antibody (1729-1828) (STJ115439)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Anti-CHD2 antibody (STJ190695)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-CHD2 IP antibody [SAA0197] (STJA0018712)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IP

Reactivity :

Human

£209.50 - £1,333.50

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Western blot analysis of extracts of various cell lines, using CHD3 rabbit monoclonal antibody (STJ11102316) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CHD3 antibody (1-100) [S6MR] (STJ11102316)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using CHD3 antibody (STJ111133) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-CHD3 antibody (1900-2000) (STJ111133)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis using Mi2-Alpha monoclonal antibody against HeLa (1) , K562 (2) , Jurkat (3) , NTERA-2 (4) , HEK293 (5) , Raji (6) cell lysate and mouse brain (7) tissue lysate.

Anti-CHD3 antibody [2G4] (STJ98244)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse

£149.00 - £360.00

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Anti-CHD3 IP antibody [SAA0258] (STJA0018773)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IP

Reactivity :

Human

£209.50 - £1,333.50

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Immunofluorescence analysis of U20S cells using CHD4 antibody (STJ113182) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CHD4 antibody (1520-1690) (STJ113182)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded mouse brain using CHD4 Rabbit monoclonal antibody (STJ11105141) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CHD4 antibody (1520-1700 aa) [S5141RM] (STJ11105141)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of HepG2 cells, using CHD4 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-CHD4 antibody (571-620 aa) (STJ94119)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-CHD4 antibody [PT1148R] (STJA0040233)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

£150.00 - £540.00

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Anti-CHD4 IP antibody [SAA0262] (STJA0018777)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IP

Reactivity :

Human

£209.50 - £1,333.50

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Anti-CHD5 antibody (25-75 aa) (STJ190696)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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STJ71152 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-CHD5 antibody (Internal) (STJ71152)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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Anti-CHD7 antibody (Internal) (STJ71285)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human/Mouse/Rat

£312.50

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Anti-CHD7 Protein-strain rsb6256 antibody [P004] (STJA0019747)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IP

Reactivity :

HRSV-A

£212.50 - £1,184.50

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Western blot analysis of various lysates using CHD9 Rabbit polyclonal antibody (STJ114040) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CHD9 antibody (85-190) (STJ114040)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Immunohistochemistry analysis of CHDH in paraffin-embedded human kidney using CHDH Rabbit polyclonal antibody (STJ118972) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CHDH antibody (245-594) (STJ118972)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-CHDH antibody [SRM13494] (STJ11108224)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of various lysates, using Chk1 Rabbit polyclonal antibody (STJ11104296) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer:3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: Q60s.

Anti-CHEK1 antibody (148-307) (STJ11104296)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Immunofluorescence analysis of PC-12 cells using Chk1 Rabbit polyclonal antibody (STJ29967) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

Anti-CHEK1 antibody (148-307) (STJ29967)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of K-562 cells, using Chk1 antibody (STJ11104686) at1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-CHEK1 antibody (250-350) [S4686RM] (STJ11104686)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £554.50

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Western blot analysis of MCF7 cells using Chk1 Polyclonal Antibody

Anti-CHEK1 antibody (286-335 aa) (STJ92267)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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STJ71518 (2µg/ml) staining of Rat Thymus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CHEK1 antibody (Internal) (STJ71518)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Cow

£312.50

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Western blot analysis using Chk1 monoclonal antibody against A431 (1) , HeLa (2) , NIH/3T3 (3) and K562 (4) cell lysate.

Anti-CHEK1 antibody [2G1D5] (STJ97955)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£149.00 - £360.00

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Western blot analysis of HeLa, using Chk2 antibody (STJ11101688) at 1:7000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CHEK2 antibody (1-100) [S8MR] (STJ11101688)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of various lysates, using Chek2 Rabbit polyclonal antibody (STJ11105188) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Chek2 antibody (1-215 aa) (STJ11105188)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Immunoprecipitation analysis of 200 Mu g extracts of MCF-7 cells using 1 Mu g Chk2 antibody (STJ114888). Western blot was performed from the immunoprecipitate using Chk2 antibody (STJ114888) at a dilution of 1:1000.

Anti-CHEK2 antibody (1-220) (STJ114888)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human

£110.50 - £505.50

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Western blot analysis of Chk2 Polyclonal Antibody, using Hela, HCT116, SW480, HEK293 cell, 4°C over night, secondary antibody (cat： (NA was diluted at 1:10000, 37°C 1hour.

Anti-CHEK2 antibody (35-84 aa) (STJ92273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis using Chk2 monoclonal antibody against cell lysate from various cell types.

Anti-CHEK2 antibody (481-531 aa) [1C12B8] (STJ97956)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£149.00 - £360.00

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Anti-Chemerin antibody (STJ11108317)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£0.00

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Western blot analysis of various lysates using CHERP Rabbit pAb (STJ111795) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-CHERP antibody (650-750) (STJ111795)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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