• Immunohistochemistry analysis of CHCHD4 in paraffin-embedded human liver cancer tissue using CHCHD4 Rabbit polyclonal antibody (STJ11105536) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CHCHD4 in paraffin-embedded rat kidney tissue using CHCHD4 Rabbit polyclonal antibody (STJ11105536) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using CHCHD4 Rabbit polyclonal antibody (STJ11105536) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-CHCHD4 antibody (1-105) (STJ11105536)

SKU:
STJ11105536

£116.50 - £543.50
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Mitochondrial Intermembrane Space Import And Assembly Protein 40 (1-105) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHCHD4
Gene ID: 131474
Uniprot ID: MIA40_HUMAN
Immunogen Region: 1-105
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-105of human CHCHD4 (NP_001091972.1).
Immunogen Sequence: MSYCRQEGKDRIIFVTKEDH ETPSSAELVADDPNDPYEEH GLILPNGNINWNCPCLGGMA SGPCGEQFKSAFSCFHYSTE EIKGSDCVDQFRAMQECMQK YPDLY
Tissue Specificity Expressed in all tissues tested, suggesting an ubiquitous expression.
Post Translational Modifications Forms intrachain disulfide bridges, but exists in different redox states.
Function Central component of a redox-sensitive mitochondrial intermembrane space import machinery which is required for the biogenesis of respiratory chain complexes. Functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17, COX19, MICU1 and COA7. Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Required for the import of COA7 in the IMS. Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS. Reduced CHCHD4/MIA40 is then reoxidized by GFER/ERV1 via a disulfide relay system. Mediates formation of disulfide bond in MICU1 in the IMS, promoting formation of the MICU1-MICU2 heterodimer that regulates mitochondrial calcium uptake.
Protein Name Mitochondrial Intermembrane Space Import And Assembly Protein 40
Coiled-Coil-Helix-Coiled-Coil-Helix Domain-Containing Protein 4
Database Links Reactome: R-HSA-1268020
Cellular Localisation Mitochondrion Intermembrane Space
Alternative Antibody Names Anti-Mitochondrial Intermembrane Space Import And Assembly Protein 40 antibody
Anti-Coiled-Coil-Helix-Coiled-Coil-Helix Domain-Containing Protein 4 antibody
Anti-CHCHD4 antibody
Anti-MIA40 antibody

Information sourced from Uniprot.org

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