• Western blot analysis of extracts of various cell lines, using CHD2 antibody (STJ28168) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CHD2 antibody (1-223) (STJ28168)

SKU:
STJ28168

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CHD2 (1-223) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHD2
Gene ID: 1106
Uniprot ID: CHD2_HUMAN
Immunogen Region: 1-223
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human CHD2 (NP_001036037.1).
Immunogen Sequence: MMRNKDKSQEEDSSLHSNAS SHSASEEASGSDSGSQSESE QGSDPGSGHGSESNSSSESS ESQSESESESAGSKSQPVLP EAKEKPASKKERIADVKKMW EEYPDVYGVRRSNRSRQEPS RFNIKEEASSGSESGSPKRR GQRQLKKQEKWKQEPSEDEQ EQGTSAESEPEQKKVKARRP VPRRTVPKPRVKKQPKTQRG KRKKQDSSDEDDDDDEAPKR QTR
Function DNA-binding helicase that specifically binds to the promoter of target genes, leading to chromatin remodeling, possibly by promoting deposition of histone H3.3. Involved in myogenesis via interaction with MYOD1: binds to myogenic gene regulatory sequences and mediates incorporation of histone H3.3 prior to the onset of myogenic gene expression, promoting their expression.
Protein Name Chromodomain-Helicase-Dna-Binding Protein 2
Chd-2
Atp-Dependent Helicase Chd2
Cellular Localisation Nucleus
Binds To Myogenic Gene Promoters
Alternative Antibody Names Anti-Chromodomain-Helicase-Dna-Binding Protein 2 antibody
Anti-Chd-2 antibody
Anti-Atp-Dependent Helicase Chd2 antibody
Anti-CHD2 antibody

Information sourced from Uniprot.org

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