St John's Laboratory Ltd

Western blot analysis of extracts of various cell lines, using ERK2 antibody (STJ11101738) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MAPK1 antibody (200-300) [S8MR] (STJ11101738)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of various cells using CERKL Polyclonal Antibody

Anti-CERKL antibody (341-390 aa) (STJ92238)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using CERK Rabbit polyclonal antibody (STJ118334) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CERK antibody (C-Term) (STJ118334)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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PERK antibody western blot K562 C6 3T3 HeLa STJA0034708

Anti-PERK antibody [R09-1F9] (STJA0034708)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L. ) variety Zhonghua 11, using OsSERK2 Rabbit polyclonal antibody (STJ11104024) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Anti-OsSERK2 antibody (STJ11104024)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

O.sativa

£143.50 - £618.50

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Anti-CERK antibody (STJA0009216)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-PERK antibody [SRM15004] (STJ11109734)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Mouse pERK1/2 (Phospho Extracellular Signal Regulated Kinase 1/2) Sandwich ELISA Kit (STJE0022157)

Applications :

ELISA

Reactivity :

Mouse

£255.50 - £335.50

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Rat pERK1/2 (Phospho Extracellular Signal Regulated Kinase 1/2) Sandwich ELISA Kit (STJE0022158)

Applications :

ELISA

Reactivity :

Rat

£255.50 - £335.50

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Western blot analysis of extracts of various cell lines, using MEK1/MEK2 antibody (STJ11102175) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MAP2K1/MAP2K2 antibody (1-360) [S5MR] (STJ11102175)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from HeLa cells using Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb (STJ22236) at 1:6000 dilution. HeLa cells were treated by PMA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-Phospho-MAP2K1/MAP2K2-S217/S221 antibody (STJ22236)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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MEK1/2 antibody western blot K562 C6 3T3 HeLa STJA0034557

Anti-MEK1/2 antibody [R08-6A7] (STJA0034557)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of extracts of various cell lines, using MAP2K1/MAP2K2 antibody (STJ11100088) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-MAP2K1/MAP2K2 antibody (200-300) (STJ11100088)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Anti-MEK1/MEK2 antibody [SRM14745] (STJ11109475)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of COLO cells using VRK3 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-VRK3 antibody (Internal) (STJ96258)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Recombinant-MEK1 antibody [SM0650] (STJA0010650)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Anti-Recombinant-MEK1 antibody [SM0690] (STJA0010690)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Western blot analysis of MEK1 at dilution of 1:1000

Anti-MEK1 antibody [SM5090] (STJA0005090)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Anti-MEK1 antibody [SM5178] (STJA0005178)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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SDS-PAGE gel of recombinant human VRK3 protein under reducing conditions Coomassie blue stain

Human VRK3 protein (Recombinant) (His-Tag) (STJP017695)

Host :

E.coli

Applications :

SDS-PAGE

£158.50 - £965.50

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Human MAP3K1 protein (Recombinant) (N-His) (STJP005306)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Human IL17RD protein (Recombinant) (C-His) (STJP013531)

Host :

Mammalian Cells

Applications :

ELISA/WB

£575.50

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Human IL17RD protein (Recombinant) (C-Fc) (STJP013532)

Host :

Mammalian Cells

Applications :

ELISA/WB

£575.50

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Western blot analysis of NIH-3T3 cells using Phospho-MEK Kinase-1 (T1402) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-MAP3K1-Thr1402 antibody (1368-1417 aa) (STJ90946)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human heart tissue, using MAP3K4 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MAP3K4 antibody (1281-1330 aa) (STJ94074)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-MAP3K4 antibody [SRM13088] (STJ11107818)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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MAP3K4 Blocking Peptide for STJ501674 peptide (STJ505315)

Applications :

Immunodepletion/Immunocompetition

£260.50

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Anti-Recombinant-MAP3K4 antibody [RM2RM67] (STJA0026526)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/WB

Reactivity :

Human

£300.50 - £457.50

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MAP3K4 Positive Control for STJ501674 peptide (STJ505316)

Applications :

WB

£308.50

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Anti-Recombinant-MAP3K4 antibody [RM2RM66] (STJA0026525)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£300.50 - £457.50

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Anti-MAP3K1 antibody [EBS-T-008] (STJ16102711)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/IHC-F/IHC-P/IF/WB

Reactivity :

Human

£387.50

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Immunohistochemistry of mouse tissue with Anti-MAP3K4 primary antibody (STJ501672) at 1:50 dilution in buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 10X.

Anti-MAP3K4 antibody (1558-1608) (STJ501672)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-MAP3K4 antibody (1558-1608) {Biotin} (STJ501673)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-MAP3K1 antibody [EBS-T-008] (STJ16101082)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/WB/IHC

Reactivity :

Human

£308.50 - £1,349.50

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Immunohistochemistry analysis of MAP3K4 in paraffin-embedded human placenta using MAP3K4 Rabbit polyclonal antibody (STJ11102801) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-MAP3K4 antibody (1-100) (STJ11102801)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

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Anti-IL17RD antibody (STJ191859)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using MAP3K1 Rabbit pAb (STJ118497) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-MAP3K1 antibody (1400-1512) (STJ118497)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-MAP3K4 antibody (1558-1608) {FITC} (STJ501674)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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MAP3K4 antibody immunocytochemistry HeLa cells STJA0035260

Anti-MAP3K4 antibody [R05-8A5] (STJA0035260)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of lysates from wild type (WT) and MAP3K1 knockout (KO) HeLa cells, using [KO Validated] MAP3K1 Rabbit polyclonal antibody (STJ11100014) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-MAP3K1 antibody (1400-1512) (STJ11100014)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Human MAP2K2 protein (Recombinant) (N-His) (STJP004642)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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MAP2K1 Blocking Peptide for STJ501657 peptide (STJ505439)

Applications :

Immunodepletion/Immunocompetition

£260.50

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Western blot phosphorylated MEK-1 Thr292 in wild type and T292A mutant samples STJA0003684

Anti-Phospho-MAP2K1-Thr292 antibody (STJA0003684)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Bovine/Canine/Non-Human Primates/Rat

£488.50

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Western blot MEK 1 phospho-Thr386 immunolabeling in T47D cells with phosphatase control STJA0003685

Anti-Phospho-MAP2K1-Thr386 antibody (STJA0003685)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Bovine/Canine/Chicken/Non-Human Primates/Mouse/Xenopus

£488.50

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Anti-Recombinant-Phospho-MAP2K1/MEK1-Thr292 antibody [RM2C23] (STJA0025195)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Rat

£300.50 - £457.50

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Phospho-MEK1 antibody western blot C6 3T3 cells STJA0034556

Anti-Phospho-MEK1-Thr292 antibody [R01-5K8] (STJA0034556)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Rat

£133.50 - £281.50

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Phospho-MEK1 antibody western blot HeLa cells Nocodazole STJA0035668

Anti-Phospho-MEK1-Thr292 antibody [R52-4S-1] (STJA0035668)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Human MAP2K2 protein (Recombinant-Active) (N-GST) (STJP011977)

Host :

Insect Cells

Applications :

ELISA/WB

£503.50

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