Western blot analysis of lysates from HeLa cells using Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb (STJ22236) at 1:6000 dilution. HeLa cells were treated by PMA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25  Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-Phospho-MAP2K1/MAP2K2-S217/S221 antibody (STJ22236)

⇓ Datasheet ⇓ SDS Citations
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ22236
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General Information

Short DescriptionRabbit polyclonal Phospho-MAP2K1/MAP2K2-S217/S221 antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:2000-1:7000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolMAP2K1
MAP2K2
Gene ID5604
5605
Uniprot IDMP2K1_HUMAN
MP2K2_HUMAN
Immunogen SequenceSMANS
SpecificityA synthetic phosphorylated peptide around S217 & S221 of human MAP2K1/MAP2K2MAP2K1 (NP_002746.1).

Additional Info

Tissue Specificity
Alternative Antibody Names Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 1 antibody
Anti-Map Kinase Kinase 1 antibody
Anti-Mapkk 1 antibody
Anti-Mkk1 antibody
Anti-Erk Activator Kinase 1 antibody
Anti-Mapk/Erk Kinase 1 antibody
Anti-Mek 1 antibody
Anti-MAP2K1 antibody
Anti-MEK1 antibody
Anti-PRKMK1 antibody
Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 2 antibody
Anti-Map Kinase Kinase 2 antibody
Anti-Mapkk 2 antibody
Anti-Erk Activator Kinase 2 antibody
Anti-Mapk/Erk Kinase 2 antibody
Anti-Mek 2 antibody
Anti-MAP2K2 antibody
Anti-MEK2 antibody
Anti-MKK2 antibody
Anti-PRKMK2 antibody

Information sourced from Uniprot.org

Citations

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