• Immunohistochemistry analysis of MAP3K4 in paraffin-embedded human placenta using MAP3K4 Rabbit polyclonal antibody (STJ11102801) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MAP3K4 in paraffin-embedded human colon carcinoma using MAP3K4 Rabbit polyclonal antibody (STJ11102801) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MAP3K4 in paraffin-embedded human lung cancer using MAP3K4 Rabbit polyclonal antibody (STJ11102801) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MAP3K4 Rabbit polyclonal antibody (STJ11102801) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MAP3K4 antibody (1-100) (STJ11102801)

SKU:
STJ11102801

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MAP3K4 (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP3K4
Gene ID: 4216
Uniprot ID: M3K4_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP3K4 (NP_005913.2).
Immunogen Sequence: MREAAAALVPPPAFAVTPAA AMEEPPPPPPPPPPPPEPET ESEPECCLAARQEGTLGDSA CKSPESDLEDFSDETNTENL YGTSPPSTPRQMKRMSTKHQ
Tissue Specificity Expressed at high levels in heart, placenta, skeletal muscle and pancreas, and at lower levels in other tissues.
Function Component of a protein kinase signal transduction cascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6.
Protein Name Mitogen-Activated Protein Kinase Kinase Kinase 4
Map Three Kinase 1
Mapk/Erk Kinase Kinase 4
Mek Kinase 4
Mekk 4
Cellular Localisation Cytoplasm
Perinuclear Region
Localized In Perinuclear Vesicular-Like Structures
Probably Golgi-Associated Vesicles
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase Kinase Kinase 4 antibody
Anti-Map Three Kinase 1 antibody
Anti-Mapk/Erk Kinase Kinase 4 antibody
Anti-Mek Kinase 4 antibody
Anti-Mekk 4 antibody
Anti-MAP3K4 antibody
Anti-KIAA0213 antibody
Anti-MAPKKK4 antibody
Anti-MEKK4 antibody
Anti-MTK1 antibody

Information sourced from Uniprot.org

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