Western blot analysis of extracts of various cell lines, using MAP2K1/MAP2K2 antibody (STJ11100088) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-MAP2K1/MAP2K2 antibody (200-300) (STJ11100088)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11100088
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General Information

Short DescriptionRabbit polyclonal MEK1/MEK2 (200-300) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityMouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolMAP2K2
MAP2K1
Gene ID5605
5604
Uniprot IDMP2K2_HUMAN
MP2K1_HUMAN
Immunogen Region200-300
Immunogen SequenceSRGEIKLCDFGVSGQLIDSM ANSFVGTRSYMSPERLQGTH YSVQSDIWSMGLSLVEMAVG RYPIPPPDAKELELMFGCQV EGDAAETPPRPRTPGRPLSS Y
SpecificityA synthetic peptide corresponding to a sequence within amino acids 200-300 of human MAP2K1 (NP_002746.1).

Additional Info

Tissue Specificity P36507 - NA
Q02750 - Widely expressed, with extremely low levels in brain.
Alternative Antibody Names Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 2 antibody
Anti-Map Kinase Kinase 2 antibody
Anti-Mapkk 2 antibody
Anti-Erk Activator Kinase 2 antibody
Anti-Mapk/Erk Kinase 2 antibody
Anti-Mek 2 antibody
Anti-MAP2K2 antibody
Anti-MEK2 antibody
Anti-MKK2 antibody
Anti-PRKMK2 antibody
Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 1 antibody
Anti-Map Kinase Kinase 1 antibody
Anti-Mapkk 1 antibody
Anti-Mkk1 antibody
Anti-Erk Activator Kinase 1 antibody
Anti-Mapk/Erk Kinase 1 antibody
Anti-Mek 1 antibody
Anti-MAP2K1 antibody
Anti-MEK1 antibody
Anti-PRKMK1 antibody

Information sourced from Uniprot.org

Citations

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