St John's Laboratory Ltd

Immunohistochemistry analysis of paraffin-embedded mouse brain using UBL3 antibody (STJ26534) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-UBL3 antibody (1-117) (STJ26534)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from wild type (WT) and UBE2C knockout (KO) HeLa cells, using [KO Validated] UBE2C Rabbit polyclonal antibody (STJ11100048) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-UBE2C antibody (1-179) (STJ11100048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£126.50 - £618.50

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Anti-UBE2C antibody [SRM14772] (STJ11109502)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of various lysates, using UCHL5 Rabbit pAb (STJ110285) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 15s.

Anti-UCHL5 antibody (1-328) (STJ110285)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates, using RNF220 Rabbit polyclonal antibody (STJ118298) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-RNF220 antibody (267-566) (STJ118298)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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SDS-PAGE gel of recombinant human PELI2 protein under reducing conditions Coomassie blue stain

Human PELI2 protein (Recombinant) (His-Tag) (STJP018888)

Host :

E.coli

Applications :

SDS-PAGE

£134.50 - £738.50

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SDS-PAGE gel of recombinant human RNF114 protein under reducing conditions with Coomassie blue stain

Human RNF114 protein (Recombinant) (His-Tag) (STJP018700)

Host :

E.coli

Applications :

SDS-PAGE/Denatured

£134.50 - £738.50

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Western blot analysis of various lysates using TRIM39 Rabbit pAb (STJ112018) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-TRIM39 antibody (350-450) (STJ112018)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Anti-MDM2 antibody [SQab22259] (STJ16103008)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£708.50

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Immunofluorescence analysis of C6 cells using Beta-TrCP/BTRC Rabbit polyclonal antibody (STJ22845) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-BTRC antibody (1-120) (STJ22845)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-FBXO4 antibody (Internal) (STJ72184)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human/Mouse/Rat/Cow

£312.50

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SDS-PAGE gel of recombinant human IPP-3 PPP1R11 protein under reducing conditions stained with Coomassie blue

Human IPP-3/PPP1R11 protein (Recombinant) (His-Tag) (STJP018279)

Host :

E.coli

Applications :

SDS-PAGE

£158.50 - £965.50

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SDS-PAGE of recombinant human RCHY1 protein under reducing conditions with Coomassie blue stain

Human RCHY1 protein (Recombinant) (His-Tag) (STJP018293)

Host :

E.coli

Applications :

SDS-PAGE/Denatured

£158.50 - £965.50

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STJ72185 (0. 5µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-FBXO4 antibody (Internal) (STJ72185)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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UCHL5 antibody western blot rat spleen STJA0034975

Anti-UCHL5 antibody [R03-9C1] (STJA0034975)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-TRIM9 antibody (Internal Region) (STJ13102488)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human

£322.50

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Western blot analysis of K-562, using YOD1 antibody (STJ115235) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-YOD1 antibody (1-348) (STJ115235)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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N2a overexpressing Mouse Znrf1 (mock transfection in first lane) and probed with STJ72828 (1µg/ml) , also staining of Mouse Brain lysates (Embryo E14 and adult cerebellum). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ZNRF1-Mouse antibody (Internal) (STJ72828)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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Western blot analysis of lysates from VEC cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-BTRC antibody (43-93 aa) (STJ195766)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of extracts of Rat testis, using Beta-TrCP/BTRC Rabbit monoclonal antibody antibody (STJ11104611) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BTRC antibody (1-100) [S4611RM] (STJ11104611)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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STJ70360 (1µg/ml) staining of Human Skeletal Muscle lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Anti-GNIP/TRIM7 antibody (C-Term) (STJ70360)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

£312.50

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Western blot analysis of extracts of various cell lines, using Uhrf2 antibody (STJ115606) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-UHRF2 antibody (76-359) (STJ115606)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using AMFR antibody (STJ22602) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AMFR antibody (384-643) (STJ22602)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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STJ72105 (1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-RNF7 antibody (Internal) (STJ72105)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse

£312.50

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Human CBL protein (Recombinant) (N-His-KSI) (STJP014449)

Host :

E.coli

Applications :

ELISA/WB

£333.50

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Mouse HMGCR (3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase) Sandwich ELISA Kit (STJE0009478)

Applications :

ELISA

Reactivity :

Mouse

£432.50 - £601.50

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RNF7 antibody western blot HepG2 cells STJA0035602

Anti-RNF7 antibody [R24-5V-6] (STJA0035602)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of various lysates using USP36 Rabbit polyclonal antibody (STJ115081) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-USP36 antibody (1-120) (STJ115081)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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STJ72280 (1µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-FANCL antibody (Internal) (STJ72280)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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STJ72567 (1µg/ml) staining of Mouse fetal (A) and adult (B) Brain lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-MKRN1 antibody (105-118) (STJ72567)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Cow

£312.50

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STJ72251 (1µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CUL2 antibody (C-Term) (STJ72251)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Mouse SOCS2 (Suppressors Of Cytokine Signaling 2) Sandwich ELISA Kit (STJE0012260)

Applications :

ELISA

Reactivity :

Mouse

£432.50 - £601.50

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STJ70107 staining (2µg/ml) of 3T3 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Anti-CBL antibody (C-Term) (STJ70107)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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Western blot analysis of lysates from Jurkat cells, using Phospho-CBL-Y700 Rabbit pAb (STJ116400) at 1:2000 dilution or CBL antibody (STJ22913). Jurkat cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-CBL-Y700 antibody (STJ116400)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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CBL antibody immunocytochemistry K562 cells STJA0034122

Anti-CBL antibody [R01-9G1] (STJA0034122)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Rat HMGCR (3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase) Sandwich ELISA Kit Ready-To-Use Kit (STJE0015861)

Applications :

ELISA

Reactivity :

Rat

£473.50 - £659.50

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Human TRIM16 protein (Recombinant) (N-His) (STJP007875)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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STJ70554 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing endoplasmic reticulum/Golgi and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-TRAC-1/RNF125 antibody (C-Term) (STJ70554)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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STJ73118 (2µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-SYVN1 antibody (Internal) (STJ73118)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human/Rat

£312.50

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STJ72068 (0. 3µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-DTX3 antibody (Internal) (STJ72068)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Cow

£312.50

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Anti-Phospho-TRAF2-Ser11 antibody (STJ196458)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-FBXO31 antibody (17-67) (STJA0006642)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-TRAF2 antibody [PT0965R] (STJA0039830)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/FC/IP

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of various lysates using ZNF645 Rabbit polyclonal antibody (STJ11100504) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ZNF645 antibody (346-425) (STJ11100504)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Rat HMGCR (3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase) Sandwich ELISA Kit (STJE0009479)

Applications :

ELISA

Reactivity :

Rat

£451.50 - £629.50

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Human SOCS2 protein (Recombinant) (N-His) (STJP003177)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Human CUL2 protein (Recombinant) (N-His) (STJP008108)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Anti-Cullin 2 antibody [SRM13623] (STJ11108353)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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