• STJ72791 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic/vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ72791 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ72791 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-USP6 antibody (142-155) (STJ72791)

SKU:
STJ72791-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-USP6 (142-155) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:4000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: USP6
Gene ID: 9098
Uniprot ID: UBP6_HUMAN
Immunogen Region: 142-155
Accession Number: NP_004496.2
Specificity: This antibody is expected NOT to cross react to USP32 or to the TBC1D3 proteins.
Immunogen Sequence: HHIDLDVRTTLRNH
Post Translational Modifications Monubiquitinated.ubiquitination is calmodulin and calcium dependent.
Function Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation.
Protein Name Ubiquitin Carboxyl-Terminal Hydrolase 6
Deubiquitinating Enzyme 6
Proto-Oncogene Tre-2
Ubiquitin Thioesterase 6
Ubiquitin-Specific-Processing Protease 6
Cellular Localisation Cell Membrane
Cytoplasm
Endosome
Localizes To The Plasma Membrane And To Filamentous Structures Within The Cell Corresponding To Arf6 Regulated Tubular Endosomes
Activation Of Rac1 And Cdc42 Can Direct The Relocalization Of Usp6 To The Plasma Membrane In A Manner That Depends On The Integrity Of The Actin Cytoskeleton
Alternative Antibody Names Anti-Ubiquitin Carboxyl-Terminal Hydrolase 6 antibody
Anti-Deubiquitinating Enzyme 6 antibody
Anti-Proto-Oncogene Tre-2 antibody
Anti-Ubiquitin Thioesterase 6 antibody
Anti-Ubiquitin-Specific-Processing Protease 6 antibody
Anti-USP6 antibody
Anti-HRP1 antibody
Anti-TRE2 antibody

Information sourced from Uniprot.org

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