Anti-Makorin antibody (Internal) (STJ70705)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-Makorin (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Dog |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:8000.WB: In transfected HEK293 transiently expressing full-length Human MKRN1 (myc and DYKDDDDK tagged) , a band of approx. 60kDa was observed. No bands were observed in mock-transfected HEK293 |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | MKRN1 |
| Gene ID | 23608 |
| Uniprot ID | MKRN1_HUMAN |
| Accession Number | NP_038474.1; NP_001138597.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | VTKEESEKEQTAVET |
| Specificity | This antibody may cross react with MKRN4 (52.9kDa). |
Additional Info
| Post Translational Modifications | Auto-ubiquitinated.which leads to proteasomal degradation. |
| Function | E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription. |
| Protein Name | E3 Ubiquitin-Protein Ligase Makorin-1Ring Finger Protein 61Ring-Type E3 Ubiquitin Transferase Makorin-1 |
| Database Links | Reactome: R-HSA-198323Reactome: R-HSA-8948751Reactome: R-HSA-983168 |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Makorin-1 antibodyAnti-Ring Finger Protein 61 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Makorin-1 antibodyAnti-MKRN1 antibodyAnti-RNF61 antibody |
Information sourced from Uniprot.org