Human UCH-L5/UCH37 protein (Recombinant) (His-Tag) (STJP017204)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human UCH-L5/UCH37-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE/Enzyme Activity |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.5 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 30% Glycerol, 200mM NaCl, 0.1mM PMSF, 2mM EDTA |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Specific activity: > 500pmol/min/ug. Measured by the hydrolysis of ubiquitin-AMC at pH 8.0, at 37C. |
Target Information
| Gene Symbol | UCHL5 |
| Gene ID | 51377 |
| Uniprot ID | UCHL5_HUMAN |
| Accession Number | NP_057068 |
| Immunogen | Human |
| Immunogen Region | 1-329aa |
| Immunogen Sequence | MGSSHHHHHH SSGLVPRGSH MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDTKTSAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWISAV RPVIEKRI |
Additional Info
| Function | Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex.however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L5Uch-L5Ubiquitin C-Terminal Hydrolase Uch37Ubiquitin Thioesterase L5 |
| Database Links | Reactome: R-HSA-2173788Reactome: R-HSA-5689603 |
| Cellular Localisation | CytoplasmNucleusAssociates With The Proteasome 19s Subunit In The CytoplasmAssociates With The Ino80 Complex In The Nucleus |
| Alternative Protein Names | Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L5 proteinUch-L5 proteinUbiquitin C-Terminal Hydrolase Uch37 proteinUbiquitin Thioesterase L5 proteinUCHL5 proteinUCH37 proteinAD-019 proteinCGI-70 protein |
Information sourced from Uniprot.org