St John's Laboratory Ltd

Western blot analysis of lysates from wild type (WT) and ITCH knockout (KO) 293T cells, using [KO Validated] ITCH Rabbit polyclonal antibody (STJ118061) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ITCH antibody (1-155) (STJ118061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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STJ71108 (0. 1µg/ml) staining of Human Brain (Frontal Cortex) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ITCH/AIF4 antibody (Internal) (STJ71108)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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ITCH/AIP4 Blocking Peptide peptide (STJ504270)

Applications :

Immunodepletion/Immunocompetition

£260.50

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ITCH antibody western blot K562 cells STJA0037252

Anti-ITCH antibody [R05-6P2] (STJA0037252)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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SDS-PAGE gel showing recombinant human USP46 UAF1 protein under reducing conditions stained with Coomassie blue

Human USP46/UAF1 protein (Recombinant) (His-Tag) (STJP018652)

Host :

E.coli

Applications :

SDS-PAGE

£172.50 - £861.50

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Western blot analysis of various lysates using RNF31 Rabbit pAb (STJ110526) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-RNF31 antibody (500-700) (STJ110526)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded human colon using Cullin 4A Rabbit monoclonal antibody (STJ11102235) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-CUL4A antibody (660-759) [S5MR] (STJ11102235)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£118.50 - £521.50

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells using 3 Mu g Cullin 4A antibody (STJ23293). Western blot was performed from the immunoprecipitate using Cullin 4A antibody (STJ23293) at a dilution of 1:1000.

Anti-CUL4A antibody (1-300) (STJ23293)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-ITCH antibody (250-300) {FITC} (STJ501508)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Immunohistochemistry of mouse muscle with Anti-UBR1 primary antibody (STJ503452) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-UBR1 antibody (1260-1500) (STJ503452)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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RAD18 antibody western blot HeLa lysates STJA0036043

Anti-Rad18 antibody [R02-5F3] (STJA0036043)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of various lysates using HACE1 Rabbit pAb (STJ117867) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-HACE1 antibody (1-100) (STJ117867)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from mouse kidney, using MKRN3 Rabbit polyclonal antibody (STJ118513) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-MKRN3 antibody (1-260) (STJ118513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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STJ72553 (0. 1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-TRIM2 antibody (233-247) (STJ72553)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Western Blot analysis mouse brain lysate demonstrating SHPRH immunolabeling STJA0005758

Anti-Shprh antibody (Internal Region) (STJA0005758)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse

£488.50

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Human OTUB2 protein (Recombinant) (N-GST) (STJP014681)

Host :

E.coli

Applications :

ELISA/WB

£503.50

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STJ71979 (1µg/ml) staining of HEK293 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-UBE2C/UBCH10 antibody (Internal) (STJ71979)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Western blot with Anti-ITCH Antibody (STJ501506) of THP cell at 1:500 dilution in buffer.

Anti-ITCH antibody (250-300) (STJ501506)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Human Atrophin-1 interacting protein 4/ITCH protein (Recombinant) (NO-Tag) (STJP020387)

Host :

E.coli

£427.50

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Human SKP1 protein (Recombinant) (N-His) (STJP006637)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Anti-UBR2 antibody (Internal) (STJ72039)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human/Mouse/Dog/Cow

£312.50

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Western blot analysis of various lysates using UBE2E1 Rabbit polyclonal antibody (STJ11101172) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-UBE2E1 antibody (1-50) (STJ11101172)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Cullin 4A antibody western blot HL-60 U2OS STJA0035171

Anti-Cullin 4A antibody [R06-0C5] (STJA0035171)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-TRIM2 antibody (162-212 aa) (STJ193894)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Recombinant-CUL4A antibody [RM2E83] (STJA0025453)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/IP/WB

Reactivity :

Human

£300.50 - £457.50

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Western blot analysis of various lysates using USP53 Rabbit polyclonal antibody (STJ116565) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-USP53 antibody (886-1073) (STJ116565)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Immunofluorescence analysis of HeLa cells using UBA1 Rabbit polyclonal antibody (STJ114237) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-UBA1 antibody (300-600) (STJ114237)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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STJ71615 (0. 05µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-OTUB1 antibody (Internal) (STJ71615)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Cow

£312.50

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CUL5 antibody western blot C6 3T3 HeLa STJA0034211

Anti-Cullin 5 antibody [R02-5K8] (STJA0034211)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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OTUB1 antibody western blot K562 rat brain C6 3T3 HeLa STJA0034667

Anti-OTUB1 antibody [R04-6I3] (STJA0034667)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of extracts of various cell lines, using Rad18 Rabbit monoclonal antibody (STJ11102044) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RAD18 antibody (1-100) [S4MR] (STJ11102044)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-UBR1 antibody (1260-1500) {FITC} (STJ503454)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Human CUL4A protein (Recombinant) (N-His-SUMO & C-Strep) (STJP007447)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Anti-USP19 antibody (Internal) (STJ71618)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA

Reactivity :

Human

£193.50

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Immunohistochemistry analysis of TRIM62 in paraffin-embedded Human colon using TRIM62 Rabbit polyclonal antibody (STJ118300) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-TRIM62 antibody (120-280) (STJ118300)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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SDS-PAGE of recombinant human RNF181 protein under reducing conditions visualized by Coomassie blue stain

Human RNF181 protein (Recombinant) (His-Tag) (STJP017826)

Host :

E.coli

Applications :

SDS-PAGE

£134.50 - £738.50

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Western blot analysis of various lysates using RNF5 Rabbit pAb (STJ110649) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-RNF5 antibody (1-120) (STJ110649)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using RNF180 Rabbit polyclonal antibody (STJ117435) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-RNF180 antibody (1-416) (STJ117435)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Human MKRN3 (Makorin Ring Finger Protein 3) Sandwich ELISA Kit Ready-To-Use Kit (STJE0017014)

Applications :

ELISA

Reactivity :

Human

£440.50 - £614.50

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STJ71093 (2. 5µg/ml) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining

Anti-RNF213/C17orf27 antibody (C-Term) (STJ71093)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human

£312.50

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Anti-RNF213/C17orf27 antibody (Internal) (STJ71092)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human

£312.50

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Immunofluorescence analysis of L929 cells using OTUB1 Rabbit polyclonal antibody (STJ113258) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-OTUB1 antibody (1-220) (STJ113258)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from Rat heart, using CUL5 Rabbit polyclonal antibody (STJ27322) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CUL5 antibody (1-300) (STJ27322)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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STJ70352 (4µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not be obtained after antigen retrieval at pH6.

Anti-EDD1/HYD antibody (C-Term) (STJ70352)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human/Mouse/Rat/Pig/Cow

£312.50

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Immunofluorescence analysis of human breast using RNF31 Polyclonal Antibody (STJ116074) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-RNF31 antibody (500-700) (STJ116074)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-RBCK1 antibody (STJA0007228)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Human RBCK1 protein (Recombinant) (N-His) (STJP007924)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Anti-OTUB1 antibody [1000] (STJA0032690)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Rat

£224.50 - £308.50

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