• Western blot analysis of various lysates using USP53 Rabbit polyclonal antibody (STJ116565) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-USP53 antibody (886-1073) (STJ116565)

SKU:
STJ116565

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-USP53 (886-1073) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: USP53
Gene ID: 54532
Uniprot ID: UBP53_HUMAN
Immunogen Region: 886-1073
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 886-1073 of human USP53 (NP_061923.2).
Immunogen Sequence: MDQCYFENSLSTECIIRSAS RSDGCQMPKLFCQNLPPPLP PKKYAITSVPQSEKSESTPD VKLTEVFKATSHLPKHSLST ASEPSLEVSTHMNDERHKET FQVRECFGNTPNCPSSSSTN DFQANSGAIDAFCQPELDSI STCPNETVSLTTYFSVDSCM TDTYRLKYHQRPKLSFPESS GFCNNSLS
Tissue Specificity Expressed predominantly in skeletal muscle and heart.
Function Tight junction-associated protein that is involved in the survival of auditory hair cells and hearing. Maybe by modulating the barrier properties and mechanical stability of tight junctions. Has no peptidase activity.
Protein Name Inactive Ubiquitin Carboxyl-Terminal Hydrolase 53
Inactive Ubiquitin-Specific Peptidase 53
Cellular Localisation Cell Junction
Tight Junction
Alternative Antibody Names Anti-Inactive Ubiquitin Carboxyl-Terminal Hydrolase 53 antibody
Anti-Inactive Ubiquitin-Specific Peptidase 53 antibody
Anti-USP53 antibody
Anti-KIAA1350 antibody

Information sourced from Uniprot.org

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