Anti-RNF5 antibody (1-120) (STJ110649)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RNF5 (1-120) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RNF5 |
| Gene ID | 6048 |
| Uniprot ID | RNF5_HUMAN |
| Immunogen Region | 1-120 |
| Immunogen Sequence | MAAAEEEDGGPEGPNRERGG AGATFECNICLETAREAVVS VCGHLYCWPCLHQWLETRPE RQECPVCKAGISREKVVPLY GRGSQKPQDPRLKTPPRPQG QRPAPESRGGFQPFGDTGGF |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human RNF5 (NP_008844.1). |
Additional Info
| Tissue Specificity | Widely expressed. |
| Function | Membrane-bound E3 ubiquitin-protein ligase that mediates ubiquitination of target proteins. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin,thereby regulating cell motility and localization of PXN/paxillin. Catalyzes ubiquitination of Salmonella type III secreted protein sopA. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD.the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of STING1 at 'Lys-150' leading to its proteasomal degradation.the ubiquitination occurs in mitochondria after viral transfection and regulates antiviral responses. Catalyzes ubiquitination and subsequent degradation of ATG4B, thereby inhibiting autophagy. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf5Ring Finger Protein 5Ram1 HomologHsrma1 |
| Database Links | Reactome: R-HSA-382556Reactome: R-HSA-5678895Reactome: R-HSA-901032 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinMitochondrion MembraneEndoplasmic Reticulum MembranePredominantly Located In The Plasma MembraneWith Some Localization Occurring Within Cytoplasmic Organelles |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf5 antibodyAnti-Ring Finger Protein 5 antibodyAnti-Ram1 Homolog antibodyAnti-Hsrma1 antibodyAnti-RNF5 antibodyAnti-G16 antibodyAnti-NG2 antibodyAnti-RMA1 antibody |
Information sourced from Uniprot.org