• Western blot analysis of extracts of various cell lines, using RNF5 antibody (STJ110649) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded mouse heart using RNF5 antibody (STJ110649) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat liver using RNF5 antibody (STJ110649) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using RNF5 antibody (STJ110649) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human liver cancer using RNF5 antibody (STJ110649) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat liver using RNF5 antibody (STJ110649) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse heart using RNF5 antibody (STJ110649) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using RNF5 antibody (STJ110649) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RNF5 antibody (1-120) (STJ110649)

SKU:
STJ110649

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RNF5 (1-120) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RNF5
Gene ID: 6048
Uniprot ID: RNF5_HUMAN
Immunogen Region: 1-120
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human RNF5 (NP_008844.1).
Immunogen Sequence: MAAAEEEDGGPEGPNRERGG AGATFECNICLETAREAVVS VCGHLYCWPCLHQWLETRPE RQECPVCKAGISREKVVPLY GRGSQKPQDPRLKTPPRPQG QRPAPESRGGFQPFGDTGGF
Tissue Specificity Widely expressed.
Function Membrane-bound E3 ubiquitin-protein ligase that mediates ubiquitination of target proteins. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin,thereby regulating cell motility and localization of PXN/paxillin. Catalyzes ubiquitination of Salmonella type III secreted protein sopA. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD.the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of STING1 at 'Lys-150' leading to its proteasomal degradation.the ubiquitination occurs in mitochondria after viral transfection and regulates antiviral responses. Catalyzes ubiquitination and subsequent degradation of ATG4B, thereby inhibiting autophagy.
Protein Name E3 Ubiquitin-Protein Ligase Rnf5
Ring Finger Protein 5
Ram1 Homolog
Hsrma1
Database Links Reactome: R-HSA-382556
Reactome: R-HSA-5678895
Reactome: R-HSA-901032
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Mitochondrion Membrane
Endoplasmic Reticulum Membrane
Predominantly Located In The Plasma Membrane
With Some Localization Occurring Within Cytoplasmic Organelles
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Rnf5 antibody
Anti-Ring Finger Protein 5 antibody
Anti-Ram1 Homolog antibody
Anti-Hsrma1 antibody
Anti-RNF5 antibody
Anti-G16 antibody
Anti-NG2 antibody
Anti-RMA1 antibody

Information sourced from Uniprot.org

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