St John's Laboratory Ltd

Western blot analysis of extracts of various cell lines, using UBE2Z antibody (STJ29305) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-UBE2Z antibody (115-354) (STJ29305)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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STJ71436 (0. 2µg/ml) staining of Human Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-TEB4/MARCH-VI antibody (701-712) (STJ71436)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse

£312.50

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Western blot analysis of extracts of various cell lines, using UBE2T antibody (STJ28935) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-UBE2T antibody (1-197) (STJ28935)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from A-549 cells, using WWP2 Rabbit polyclonal antibody (STJ26122).

Anti-WWP2 antibody (151-400) (STJ26122)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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SDS-PAGE gel of recombinant human Ubc2 UBE2A protein under reducing conditions

Human Ubc2/UBE2A protein (Recombinant) (His-Tag) (STJP016695)

Host :

E.coli

Applications :

SDS-PAGE

£125.50 - £649.50

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Anti-WWP1 antibody (N-Term) (STJA0005653)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat

£319.50

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Western blot analysis of extracts of various cell lines, using RFWD2 antibody (STJ112492) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-RFWD2 antibody (582-731) (STJ112492)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Anti-NEDD4 antibody (STJ13101434)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse

£322.50

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Western blot analysis of various lysates using TRIP12 Rabbit pAb (STJ111999) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit Exposure time: 30s.

Anti-TRIP12 antibody (1-240) (STJ111999)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Human UBE2D3 protein (Recombinant) (C-His) (STJP000786)

Host :

E.coli

£71.50 - £185.50

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Immunohistochemistry analysis of paraffin-embedded rat brain using UBE4A Rabbit polyclonal antibody (STJ26241) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-UBE4A antibody (797-1066) (STJ26241)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-COP1/RFWD2 antibody (582-731 aa) [SRM5265] (STJ11107167)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£126.50 - £554.50

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Immunoprecipitation analysis of 300 Mu g extracts of Mouse lung using 3 Mu g SMURF2 antibody (STJ11101803). Western blot was performed from the immunoprecipitate using SMURF2 antibody (STJ11101803) at a dilution of 1:1000.

Anti-SMURF2 antibody (201-290) [S3MR] (STJ11101803)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of various lysates using ARIH1 Rabbit polyclonal antibody (STJ11102851) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ARIH1 antibody (400 aa) (STJ11102851)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot WWP1 expression in mouse liver and PC3 cells using antibody STJA0041456

Anti-WWP1 antibody (N-Term) (STJA0041456)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Chicken/Human/Mouse/Rat

£474.50

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Western blot analysis of various lysates using CDC34 Rabbit mAb (STJ11102546) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-CDC34 antibody (20-236) [S6MR] (STJ11102546)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of BALB-3T3 cells using CDC34 Rabbit polyclonal antibody (STJ27410) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CDC34 antibody (150-236) (STJ27410)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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CDC34 antibody western blot Jurkat NIH/3T3 STJA0036205

Anti-CDC34 antibody [R09-8K4] (STJA0036205)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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CDC34 antibody western blot 3T3 PC-12 Raw2647 STJA0035152

Anti-CDC34 antibody [R08-6G3] (STJA0035152)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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SDS-PAGE gel showing recombinant human Ubc6 UBE2J2 protein 3µg under reducing conditions with Coomassie blue stain

Human Ubc6/UBE2J2 protein (Recombinant) (His-Tag) (STJP017717)

Host :

E.coli

Applications :

SDS-PAGE

£134.50 - £738.50

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Western blot analysis of various lysates using UBE2E2 Rabbit polyclonal antibody (STJ118869) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-UBE2E2 antibody (1-201) (STJ118869)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Western blot analysis of 293T, using MARCH9 antibody (STJ112609) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MARCH9 antibody (277-346) (STJ112609)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded human gastric using NEDD4 antibody (STJ24729) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-NEDD4 antibody (160-460) (STJ24729)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£110.50 - £505.50

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NEDD4 Blocking Peptide for STJ506278 peptide (STJ506279)

Applications :

Immunodepletion/Immunocompetition

£260.50

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Western blot analysis of extracts of Rat skeletal muscle, using WWP1 antibody (STJ117813) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-WWP1 antibody (1-260) (STJ117813)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-TEB4/MARCH-VI antibody (886-898) (STJ71362)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human/Mouse

£312.50

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HSPC150 antibody western blot HeLa lysates STJA0035633

Anti-HSPC150 antibody [R53-8D-3] (STJA0035633)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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STJ70343 (1µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-TRC8 antibody (C-Term) (STJ70343)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Dog

£312.50

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SDS-PAGE gel of recombinant human UBE2T protein under reducing conditions stained with Coomassie blue

Human UBE2T protein (Recombinant) (His-Tag) (STJP016669)

Host :

E.coli

Applications :

SDS-PAGE

£125.50 - £649.50

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Western blot analysis of extracts of various cell lines, using NEDD4 Rabbit monoclonal antibody (STJ11102065) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-NEDD4 antibody (1200-1300) [S5MR] (STJ11102065)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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NEDD4 antibody western blot K562 HCT116 3T3 C6 STJA0035032

Anti-NEDD4 antibody [R06-7C3] (STJA0035032)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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UBE4A antibody western blot 293 cells STJA0035517

Anti-UBE4A antibody [R89-5S-8] (STJA0035517)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/FC

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-UBOX5 antibody (127-177 aa) (STJ194674)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using NEDD4L Rabbit pAb (STJ110385) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-NEDD4L antibody (836-955) (STJ110385)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using Phospho-NEDD4L-S448 Rabbit pAb (STJ11100984) at 1:1000 dilution. NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. C6 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-NEDD4L-S448 antibody (STJ11100984)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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NEDD4-2 antibody western blot K562 HeLa lysates STJA0035442

Anti-NEDD4-2 antibody [R41-8P-2] (STJA0035442)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-RNF139 antibody (500-550 aa) (STJ13101959)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£322.50

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Anti-Recombinant-CUL1 antibody [RM2E77] (STJA0025447)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Immunohistochemistry analysis of UBE2B in paraffin-embedded rat brain tissue using UBE2B Rabbit monoclonal antibody (STJ11102688) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-UBE2B antibody (53-152) [S8MR] (STJ11102688)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Human UBE2J2 protein (Recombinant) (N-GST) (STJP014788)

Host :

E.coli

Applications :

ELISA/WB

£503.50

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Immunofluorescence analysis of NIH-3T3 cells using UBE2L3 Polyclonal Antibody (STJ26020) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-UBE2L3 antibody (73-212) (STJ26020)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using UBE2W Rabbit polyclonal antibody (STJ119105) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-UBE2W antibody (40-110) (STJ119105)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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STJ70308 staining (0. 5µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Anti-ARIH1/HHARI antibody (C-Term) (STJ70308)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Cow

£312.50

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SDS-PAGE gel of recombinant human UbcH5c UBE2D3 protein under reducing conditions stained with Coomassie blue

Human UbcH5c/UBE2D3 protein (Recombinant) (His-Tag) (STJP017394)

Host :

E.coli

Applications :

SDS-PAGE

£134.50 - £738.50

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Human UBE2S protein (Recombinant) (N-GST) (STJP014650)

Host :

E.coli

Applications :

ELISA/WB

£319.50

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Immunofluorescence analysis of NIH/3T3 cells using UBE2L6 antibody (STJ115626) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-UBE2L6 antibody (100-153) (STJ115626)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates, using USP24 antibody (STJ11102699) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-USP24 antibody (2500-2600) [S9MR] (STJ11102699)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of various lysates using UBE2D3 Rabbit polyclonal antibody (STJ116847) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-UBE2D3 antibody (1-100) (STJ116847)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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