Signal Transduction | Research Area | St John's Laboratory

Western blot analysis of lysates from mouse heart, using NGF Rabbit polyclonal antibody (STJ115857) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-NGF antibody (19-241) (STJ115857)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of extracts of Rat liver, using NGF antibody (STJ116148) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-NGF antibody (19-241) (STJ116148)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Anti-NGF antibody (195-240 aa) (STJ13100203)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human

£331.50

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Western blot analysis of various cells using NGF Polyclonal Antibody diluted at 1：2000

Anti-NGF antibody (33-82) (STJ94479)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using NGFR p75 Polyclonal Antibody

Anti-NGFR antibody (121-170) (STJ94481)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using (STJ113724) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NGFR antibody (273-427) (STJ113724)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Human malignant melanoma stained with anti-NGFR antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of tumor cells.

Anti-NGFR antibody (281-421aa) [ZM55] (STJ180305)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£164.50 - £601.50

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Immunohistochemistry analysis of paraffin-embedded human lung cancer using p75NTR/NGFR Rabbit monoclonal antibody (STJ11101625) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-NGFR antibody (300-400) [S5MR] (STJ11101625)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of lysates from Rat spleen using p75NTR/NGFR Rabbit polyclonal antibody (STJ24767) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:30s.

Anti-NGFR antibody (301-400) (STJ24767)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of CD271 in paraffin-embedded human tonsil tissue using CD271 Rabbit polyclonal antibody (STJ11105609) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.

Anti-NGFR antibody (330-427) (STJ11105609)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis using NGFR p75 monoclonal antibody against NGFR-hIgGFc transfected HEK293 cell lysate.

Anti-NGFR antibody [2F1C2] (STJ98277)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-NGFR antibody [MRQ-21] (STJ16100403)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£1,136.50 - £1,855.50

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Immunofluorescence analysis of MCF-7 cells using NGFRAP1 antibody (STJ29435).

Anti-NGFRAP1 antibody (1-111) (STJ29435)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-NIPSNAP2 antibody (STJA0009524)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NISCH antibody (234-284 aa) (STJ196047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-NLK antibody (230-310 aa) (STJ191357)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human placenta using NLK Rabbit monoclonal antibody (STJ11103001) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-NLK antibody (400-500) [S1MR] (STJ11103001)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of HeLa, using NLN Rabbit polyclonal antibody (STJ115078) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NLN antibody (630-704) (STJ115078)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Anti-NLN antibody (STJA0008836)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NMB antibody (20-100 aa) (STJ191072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of 293 cells using NMBR Polyclonal Antibody

Anti-NMBR antibody (221-270) (STJ94513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-NMBR antibody (STJ13100204)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC

Reactivity :

Rat/Mouse/Human/Marmoset

£331.50

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Anti-NMDAR1 antibody (ECD) (STJ13100195)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Western blot analysis of various cells using Neuromedin-S Polyclonal Antibody diluted at 1：1000

Anti-NMS antibody (104-153) (STJ94432)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using Neuromedin-U Polyclonal Antibody diluted at 1：500

Anti-NMU antibody (125-174) (STJ94433)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-NMU antibody (35-174 aa) (STJ11106826)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using NMU antibody (STJ116312) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NMU antibody (35-174) (STJ116312)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of K562 cells using NMUR1 Polyclonal Antibody

Anti-NMUR1 antibody (1-50) (STJ94524)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from SGC-7901 cells, using NMUR1 Rabbit polyclonal antibody (STJ119674) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NMUR1 antibody (360-426) (STJ119674)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of mouse-brain cells using primary antibody diluted at 1:2000 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour)

Anti-NMUR2 antibody (1-50 aa) (STJ99340)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:200

Anti-NOG antibody (21-70 aa) (STJ98814)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-NOG antibody (Internal Region) (STJA0005795)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£455.50

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Western blot detection of Noggin in CHO-K1 cell lysates over-expressing Noggin-PDGFR transmembrane domain fused protein using Noggin mouse mAb (1:1000 diluted).Predicted band size:26KDa.Observed band size:37KDa.

Anti-NOG antibody [1D2-G8-G1] (STJ99187)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of lysates from A-549 cells, using Non-phospho-YAP1-S127 Rabbit pAb (STJ11103239) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Non-phospho-YAP1-S127 antibody (50-150) (STJ11103239)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of human placenta cells using eNOS Rabbit polyclonal antibody (STJ24784) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-NOS3 antibody (1000-1203) (STJ24784)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of human placenta cells using eNOS Rabbit polyclonal antibody (STJ11104517) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-NOS3 antibody (1035-1110) (STJ11104517)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunohistochemistry analysis of eNOS in paraffin-embedded rat spleen tissue using eNOS Rabbit monoclonal antibody (STJ11104147) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-NOS3 antibody (1035-1110) [S4147RM] (STJ11104147)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunofluorescence analysis of Hela cell. 1, NOS3 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-NOS3 antibody (1145-1194) (STJ94539)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of HUVEC cells using eNOS Rabbit polyclonal antibody (STJ117269) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NOS3 antibody (639-738) (STJ117269)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-NOS3 antibody (C-Term) (STJA0005490)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£319.50

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Immunohistochemistry analysis of paraffin-embedded human lymph node (left) and colon cancer (right) tissues with DAB staining using NOS3 monoclonal antibody.

Anti-NOS3 antibody [6H2] (STJ98282)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of lysates from 1) Rat Heart Tissue, 2) huvec, 3) Jurkat cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta Polyclonal Antibody (cat: (STJ91464) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-NOS3 antibody [Mix] (STJ97066)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Rabbit

£48.00 - £252.00

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Western blot analysis of various lysates, using eNOS antibody (STJ11104808) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-NOS3 antibody [S4808RM] (STJ11104808)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of Jurkat cells, using Notch1 antibody (STJ11100238) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NOTCH1 antibody (1567-1729) (STJ11100238)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using Notch1 Rabbit pAb (STJ119092) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-NOTCH1 antibody (1715-1814) (STJ119092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using Notch1 Rabbit polyclonal antibody (STJ11103863) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NOTCH1 antibody (2381-2480) (STJ11103863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of Rat brain cells, using Notch1 antibody (STJ29951) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NOTCH1 antibody (2456-2555) (STJ29951)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 200 Mu g extracts of 293T cells using 3 Mu g Notch1 antibody (STJ11101627). Western blot was performed from the immunoprecipitate using Notch1 antibody (STJ11101627) at a dilution of 1:1000.

Anti-NOTCH1 antibody (2456-2555) [S7MR] (STJ11101627)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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