• Immunofluorescence analysis of mouse ovary using FSHR Rabbit polyclonal antibody (STJ23713) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat ovary using FSHR Rabbit polyclonal antibody (STJ23713) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa using FSHR Rabbit polyclonal antibody (STJ23713) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using FSHR antibody (STJ23713) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of SKOV3 cells, using FSHR antibody (STJ23713) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-FSHR antibody (631-695) (STJ23713)

SKU:
STJ23713

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FSHR (631-695) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FSHR
Gene ID: 2492
Uniprot ID: FSHR_HUMAN
Immunogen Region: 631-695
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 631-695 of human FSHR (NP_000136.2).
Immunogen Sequence: KNFRRDFFILLSKCGCYEMQ AQIYRTETSSTVHNTHPRNG HCSSAPRVTSGSTYILVPLS HLAQN
Tissue Specificity Sertoli cells and ovarian granulosa cells.
Post Translational Modifications Sulfated. N-glycosylated.indirectly required for FSH-binding, possibly via a conformational change that allows high affinity binding of hormone.
Function G protein-coupled receptor for follitropin, the follicle-stimulating hormone. Through cAMP production activates the downstream PI3K-AKT and ERK1/ERK2 signaling pathways.
Protein Name Follicle-Stimulating Hormone Receptor
Fsh-R
Follitropin Receptor
Database Links Reactome: R-HSA-375281
Reactome: R-HSA-418555
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Follicle-Stimulating Hormone Receptor antibody
Anti-Fsh-R antibody
Anti-Follitropin Receptor antibody
Anti-FSHR antibody
Anti-LGR1 antibody

Information sourced from Uniprot.org

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