Programmed Cell Death | Research Area | St John's Laboratory

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKC thet (Phospho-Ser676) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PRKCQ-Ser676 antibody (643-692 aa) (STJ90391)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 15', using PKC thet (Phospho-Ser695) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PRKCQ-Ser695 antibody (657-706 aa) (STJ90879)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of lysates from Jurkat and HeLa cells, using Phospho-PRKCQ-T538 Rabbit pAb (STJ117896) at 1:2000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes. HeLa cells were treated by serum-starvation overnight or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-PRKCQ-T538 antibody (STJ117896)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $853.53

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-PRKCQ-Thr538 antibody (504-553 aa) (STJ90865)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of 293T AD293 Hela using p-Parkin (S131) antibody. Antibody was diluted at 1:500

Anti-Phospho-PRKN-Ser131 antibody (101-150 aa) (STJ91122)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of 3T3 cells using Phospho-20S Proteasome Alpha 3 (S250) Polyclonal Antibody

Anti-Phospho-PSMA3-Ser250 antibody (206-255 aa) (STJ90556)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western Blot analysis of 1 MCF-7 treated with LPS, 2 MCF7, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-PTK2-Ser722 antibody (STJA0009853)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PTK2-Ser732 antibody (STJ193214)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of HELA KB 293T 3T3 lysis using Phospho-FAK (S843) antibody. Antibody was diluted at 1:1000

Anti-Phospho-PTK2-Ser843 antibody (809-858 aa) (STJ90571)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of HepG2 cells, using FAK (Phospho-Ser910) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PTK2-Ser910 antibody (876-925 aa) (STJ91306)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-PTK2-Tyr-397 antibody [M121] (STJA0005417)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Rabbit

$504.39

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Western blot analysis of lysates from Jurkat cells treated with Ca2+ 40nM 30', using FAK (Phospho-Tyr397) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PTK2-Tyr397 antibody (363-412 aa) (STJ90899)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of 293 cells using Phospho-FAK (Y397) Polyclonal Antibody

Anti-Phospho-PTK2-Tyr397 antibody (364-413 aa) (STJ90675)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various cells using Phospho-FAK (Y407) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-PTK2-Tyr407 antibody (373-422 aa) (STJ90809)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Western blot analysis of 3T3 cells using Phospho-FAK (Y576) Polyclonal Antibody

Anti-Phospho-PTK2-Tyr576 antibody (542-591 aa) (STJ90789)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using FAK (Phospho-Tyr577) Antibody

Anti-Phospho-PTK2-Tyr577 antibody (543-592 aa) (STJ91185)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various cells using Phospho-FAK (Y861) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-PTK2-Tyr861 antibody (828-877 aa) (STJ90268)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Western blot analysis of lysates from Jurkat and HepG2 cells, using FAK (Phospho-Tyr925) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PTK2-Tyr925 antibody (892-941 aa) (STJ90269)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from A-549 cells, using Phospho-FAK-Y397 Rabbit pAb (STJ27521) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-PTK2-Y397 antibody (STJ27521)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of A-549, using Phospho-FAK-Y397 Rabbit monoclonal antibody (STJ11105895) at 1:4000 dilution. A-549 cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-PTK2-Y397 antibody [S5895RM] (STJ11105895)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$163.53 - $719.67

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Western blot analysis of various lysates, using Phospho-FAK-Y576/577 Rabbit pAb (STJ117876) at 1:1000 dilution. Jurkat cells were treated by CD-3 (10 Mu g/mL) for 2 minutes after serum-starvation overnight or treated by PMA/TPA (200nM) for 10 minutes. Raji cells were treated by PMA/TPA (200nM) for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-PTK2-Y576/577 antibody (STJ117876)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $853.53

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Western blot analysis of lysates from Jurkat cells, using Phospho-FAK-Y925 Rabbit pAb (STJ29365) 1:1000 dilution.. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-PTK2-Y925 antibody (STJ29365)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $853.53

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Anti-Phospho-RIP3-S199 antibody [P328] (STJA0019148)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

$289.11 - $1,840.23

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Anti-Phospho-RIP3-S227 antibody [P2A11] (STJA0019147)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

$289.11 - $1,840.23

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Western blot analysis of HT-29 cells, using Phospho-RIPK1-S166 Rabbit monoclonal antibody (STJ11105896) at 1:4000 dilution. HT-29 cells were treated with Z-VAD (50uM) , human TNF-Alpha (50ng/ml) and BV-6 (200nM) at 37 °C for 5 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-RIPK1-S166 antibody [S5896RM] (STJ11105896)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$163.53 - $719.67

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Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-RIPK1-Ser166 antibody (STJ196402)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of lysates from HT-29 cells, using Phospho-RIPK1/RIP-S166 Rabbit pAb (STJ11103757) at 1:800 dilution. HT-29 cells were treated with Z-VAD (50uM) , human TNF-Alpha (50ng/ml) and SM-164 (200nM) at 37 °C for 5 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-RIPK1/RIP-S166 antibody (STJ11103757)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.57 - $853.53

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Anti-Phospho-RIPK3-S199 antibody [SAA0298] (STJA0019150)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

$289.11 - $1,840.23

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Anti-Phospho-RIPK3-S227 antibody [SAA0297] (STJA0019149)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

$289.11 - $1,840.23

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-RIPK3-Ser227 antibody (STJ196403)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human

$67.62 - $360.18

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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SATB1-Ser47 antibody (STJ196412)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western Blot analysis of 1 A431 cell 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-SFN-Ser186 antibody (STJA0009865)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot with Anti-Phospho-STAT3 Antibody (STJ502696) of THP cells. Antibody at 1:500 dilution in buffer.

Anti-Phospho-STAT3 antibody (700-750) (STJ502696)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$729.33

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Anti-Phospho-STAT3 antibody (700-750) {Biotin} (STJ502697)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$806.61

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Anti-Phospho-STAT3 antibody (700-750) {FITC} (STJ502698)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$806.61

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Western blot analysis of lysates from HeLa cells, using Phospho-STAT3-S727 Rabbit pAb (STJ116371) at 1:1000 dilution. HeLa cells were treated by UV at room temperature for 15-30 minutes. HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-STAT3-S727 antibody (STJ116371)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of various lysates using Phospho-STAT3-S727 Rabbit mAb (STJ11102565) at 1:1000 dilution. NIH/3T3 and PC-12 cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 120s.

Anti-Phospho-STAT3-S727 antibody [S5MR] (STJ11102565)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Immunofluorescence analysis of Hela cell. 1, Stat3 (phospho Ser727) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase 9 monoclonal antibody (3-20) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-STAT3-Ser727 antibody (694-743 aa) (STJ90410)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/IP/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Anti-Phospho-STAT3-Ser727 antibody [PT0911R] (STJA0039802)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-STAT3-Ser754 antibody (STJ196440)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

$67.62 - $360.18

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Immunofluorescence analysis of mouse-spleen tissue. 1, Stat3 (phospho Tyr705) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-STAT3-Tyr705 antibody (672-721 aa) (STJ90411)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/IP/ELISA

Reactivity :

Human/Mouse/Rat/Pig

$67.62 - $360.18

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Anti-Phospho-STAT3-Tyr705 antibody [PT0742R] (STJA0039211)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using Muscle Actin Mouse mAb diluted at 1:200.

Anti-Phospho-STAT3-Y705 antibody (640-720 aa) [1E9] (STJ97747)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of lysates from HeLa cells, using Phospho-STAT3-Y705 Rabbit pAb (STJ27511) at 1:500 dilution. HeLa cells were treated by IFN-Alpha (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-STAT3-Y705 antibody (STJ27511)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-STAT3-Y705 Rabbit mAb (STJ11102563) at 1:1000 dilution. NIH/3T3 cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-STAT3-Y705 antibody [S3MR] (STJ11102563)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$198.03 - $853.53

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Immunohistochemistry analysis of paraffin-embedded rat kidney using Phospho-STAT3-Y705 Rabbit monoclonal antibody (STJ11105819) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-STAT3-Y705 antibody [S5819RM] (STJ11105819)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$198.03 - $853.53

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Anti-Phospho-STAT3-Y705 antibody [SAA0302] (STJA0019171)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

$289.11 - $1,840.23

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Anti-Phospho-STAT3-Y705-Y705 antibody [SRM15135] (STJ11109865)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

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