Anti-OCLN antibody (500aa C-Term) (STJ140166)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
ImmunogenPeptide derived from within residues 500 aa to the C-terminus of human Occludin.
General Information
| Short Description | Goat polyclonal Occludin (500aa C-Term) antibody for use in WB, IHC-F and IHC-P in human, rat, mouse, canine and monkey samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-F/IHC-P |
| Host | Goat |
| Reactivity | Human/Rat/Mouse/Canine/Monkey |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 3 mg/mL |
| Purification | This antibody is epitope-affinity purified from goat antiserum. |
| Dilution Range | WB 1:500-1:2000IHC-F 1:250-1:1000IHC-P 1:250-1:1000 |
| Formulation | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Storage Instruction | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Target Information
| Gene Symbol | OCLN |
| Gene ID | 100506658 |
| Uniprot ID | OCLN_HUMAN |
| Accession Number | ENSG00000197822 |
| Immunogen | Peptide derived from within residues 500 aa to the C-terminus of human Occludin. |
| Immunogen Region | 500aa C-Term |
| Immunogen Sequence | CKQLKSKLSHIKKMVGDYDR KQLKSKLSHIKKMVGDYDRQ |
| Specificity | Detects endogenous levels of OCLN in MCR, IMCD3, COS7, MDCK, HUH, HEK293, MCF7, mouse brain by Western blot. |
Additional Info
| Tissue Specificity | Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. |
| Post Translational Modifications | Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. Phosphorylation at Ser-490 also attenuates the interaction with TJP1. (Microbial infection) Cleaved by S.pyogenes SpeB protease.leading to its degradation. Degradation by SpeB promotes bacterial translocation across the host epithelial barrier. |
| Function | May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. (Microbial infection) Acts as a coreceptor for hepatitis C virus (HCV) in hepatocytes. |
| Protein Name | Occludin |
| Database Links | Reactome: R-HSA-351906Reactome: R-HSA-8935964 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCell JunctionTight Junction |
| Alternative Antibody Names | Anti-Occludin antibodyAnti-OCLN antibody |
Information sourced from Uniprot.org