Anti-OPA1 antibody (STJ193080)

SKU:
STJ193080

Current Stock:
Host: Rabbit
Applications: WB, ELISA
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal anti-OPA1 antibody is suitable for use in Western Blot and ELISA.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Purification: OPA1 antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-2000
ELISA 1:5000-20000
Specificity: OPA1 polyclonal antibody detects endogenous levels of protein.
Uniprot ID: OPA1_HUMAN
Gene ID: 4976
Gene Symbol: OPA1
Post Translational Modifications PARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion. Cleaved by OMA1 at position S1 following stress conditions. Isoform 2: Cleavage at position S2 is mediated by YME1L. Cleavage may occur in the sequence motif Leu-Gln-Gln-Gln-Ile-Gln (LQQQIQ). Isoform 3: Cleavage at position S2 is mediated by YME1L. Cleavage may occur in the sequence motif Leu-Gln-Gln-Gln-Ile-Gln (LQQQIQ). Isoform 4: Cleavage at position S2 is mediated by YME1L. Cleavage may occur in the sequence motif Leu-Gln-Gln-Gln-Ile-Gln (LQQQIQ).
Function Dynamin-related GTPase that is essential for normal mitochondrial morphology by regulating the equilibrium between mitochondrial fusion and mitochondrial fission. Coexpression of isoform 1 with shorter alternative products is required for optimal activity in promoting mitochondrial fusion. Binds lipid membranes enriched in negatively charged phospholipids, such as cardiolipin, and promotes membrane tubulation. The intrinsic GTPase activity is low, and is strongly increased by interaction with lipid membranes. Plays a role in remodeling cristae and the release of cytochrome c during apoptosis. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space. Plays a role in mitochondrial genome maintenance. Dynamin-like 120 kDa protein, form S1: Inactive form produced by cleavage at S1 position by OMA1 following stress conditions that induce loss of mitochondrial membrane potential, leading to negative regulation of mitochondrial fusion. Isoforms that contain the alternative exon 4b (present in isoform 4 and isoform 5) are required for mitochondrial genome maintenance, possibly by anchoring the mitochondrial nucleoids to the inner mitochondrial membrane.
Protein Name Dynamin-Like 120 Kda Protein - Mitochondrial
Optic Atrophy Protein 1 Cleaved Into - Dynamin-Like 120 Kda Protein - Form S1
Database Links Reactome: R-HSA-169911
Cellular Localisation Mitochondrion Inner Membrane
Single-Pass Membrane Protein
Mitochondrion Intermembrane Space
Mitochondrion Membrane
Detected At Contact Sites Between Endoplasmic Reticulum And Mitochondrion Membranes
Alternative Antibody Names Anti-Dynamin-Like 120 Kda Protein - Mitochondrial antibody
Anti-Optic Atrophy Protein 1 Cleaved Into - Dynamin-Like 120 Kda Protein - Form S1 antibody
Anti-OPA1 antibody
Anti-KIAA0567 antibody

Information sourced from Uniprot.org

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