• Western blot analysis of extracts of various cell lines, using NMT1 antibody (STJ111389) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-NMT1 antibody (1-250) (STJ111389)

SKU:
STJ111389

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NMT1 (1-250) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NMT1
Gene ID: 4836
Uniprot ID: NMT1_HUMAN
Immunogen Region: 1-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NMT1 (NP_066565.1).
Immunogen Sequence: MADESETAVKPPAPPLPQMM EGNGNGHEHCSDCENEEDNS YNRGGLSPANDTGAKKKKKK QKKKKEKGSETDSAQDQPVK MNSLPAERIQEIQKAIELFS VGQGPAKTMEEASKRSYQFW DTQPVPKLGEVVNTHGPVEP DKDNIRQEPYTLPQGFTWDA LDLGDRGVLKELYTLLNENY VEDDDNMFRFDYSPEFLLWA LRPPGWLPQWHCGVRVVSSR KLVGFISAIPANIHIYDTE
Function Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins. Also able to mediate N-terminal lysine myristoylation of proteins: catalyzes myristoylation of ARF6 on both 'Gly-2' and 'Lys-3'. Lysine myristoylation is required to maintain ARF6 on membranes during the GTPase cycle.
Protein Name Glycylpeptide N-Tetradecanoyltransferase 1
Myristoyl-Coa -Protein N-Myristoyltransferase 1
Hsnmt1
Nmt 1
Type I N-Myristoyltransferase
Peptide N-Myristoyltransferase 1
Protein-Lysine Myristoyltransferase Nmt1
Database Links Reactome: R-HSA-162599
Reactome: R-HSA-2514859
Reactome: R-HSA-75108
Cellular Localisation Cytoplasm
Cytosol
Membrane
Peripheral Membrane Protein
Copurifies With Ribosomes
Alternative Antibody Names Anti-Glycylpeptide N-Tetradecanoyltransferase 1 antibody
Anti-Myristoyl-Coa -Protein N-Myristoyltransferase 1 antibody
Anti-Hsnmt1 antibody
Anti-Nmt 1 antibody
Anti-Type I N-Myristoyltransferase antibody
Anti-Peptide N-Myristoyltransferase 1 antibody
Anti-Protein-Lysine Myristoyltransferase Nmt1 antibody
Anti-NMT1 antibody
Anti-NMT antibody

Information sourced from Uniprot.org

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