• Western blot analysis of extracts of various cell lines, using Neutrophil Elastase (ELANE) antibody (STJ11103639) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunofluorescence analysis of paraffin-embedded human spleen using Neutrophil Elastase (ELANE) Rabbit monoclonal antibody (STJ11103639) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded mouse bone marrow using Neutrophil Elastase (ELANE) antibody (STJ11103639) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Neutrophil Elastase (ELANE) in paraffin-embedded Mouse bone marrow using Neutrophil Elastase (ELANE) Rabbit monoclonal antibody (STJ11103639) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of human spleen using Neutrophil Elastase (ELANE) rabbit monoclonal antibody (STJ11103639) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using Neutrophil Elastase (ELANE) Rabbit monoclonal antibody (STJ11103639) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-Neutrophil Elastase antibody (167-267) [S9MR] (STJ11103639)

SKU:
STJ11103639

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Neutrophil Elastase (ELANE) (167-267) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ELANE
Gene ID: 1991
Uniprot ID: ELNE_HUMAN
Immunogen Region: 167-267
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 167-267 of human Neutrophil Elastase (ELANE) (P08246).
Immunogen Sequence: SVLQELNVTVVTSLCRRSNV CTLVRGRQAGVCFGDSGSPL VCNGLIHGIASFVRGGCASG LYPDAFAPVAQFVNWIDSII QRSEDNPCPHPRDPDPASRT H
Tissue Specificity Bone marrow cells. Neutrophil.
Function Serine protease that modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Promotes cleavage of GSDMB, thereby inhibiting pyroptosis. Capable of killing E.coli but not S.aureus in vitro.digests outer membrane protein A (ompA) in E.coli and K.pneumoniae.
Protein Name Neutrophil Elastase
Bone Marrow Serine Protease
Elastase-2
Human Leukocyte Elastase
Hle
Medullasin
Pmn Elastase
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474228
Reactome: R-HSA-1592389
Reactome: R-HSA-5620971
Reactome: R-HSA-6798695
Reactome: R-HSA-6803157
Reactome: R-HSA-977606
Cellular Localisation Cytoplasmic Vesicle
Phagosome
Localized In Phagolysosomes Following Ingestion Of E
Coli By Neutrophils
Alternative Antibody Names Anti-Neutrophil Elastase antibody
Anti-Bone Marrow Serine Protease antibody
Anti-Elastase-2 antibody
Anti-Human Leukocyte Elastase antibody
Anti-Hle antibody
Anti-Medullasin antibody
Anti-Pmn Elastase antibody
Anti-ELANE antibody
Anti-ELA2 antibody

Information sourced from Uniprot.org

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