Programmed Cell Death | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using Phospho-Tau-T231 rabbit monoclonal antibody (STJ11102551) at 1:1000 dilution. HeLa and NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-Phospho-MAPT-T231 antibody [S1MR] (STJ11102551)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse/Rat

£146.50 - £642.50

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Western blot analysis of extracts of HeLa cells, using Phospho-Tau-T181 antibody (STJ11101032) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-Phospho-MAPT-T498 antibody (STJ11101032)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of 293 cells using Phospho-Tau (T181) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-MAPT-Thr181 antibody (471-520 aa) (STJ90425)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of VEC 293 lysis using Phospho-Tau (T205) antibody. Antibody was diluted at 1:2000

Anti-Phospho-MAPT-Thr205 antibody (491-540 aa) (STJ90426)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using Tau (Phospho-Thr212) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MAPT-Thr212 antibody (496-545 aa) (STJ91285)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of mouse brain, using Phospho-Tau-Thr212/Ser214 antibody (STJ11101159) at 1:1000 dilution. mouse brain were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-MAPT-Thr212/Ser214 antibody (STJ11101159)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of extracts of mouse brain, using Phospho-Tau-Thr231 antibody (STJ11101057) at 1:1000 dilution. mouse brain were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-MAPT-Thr231 antibody (STJ11101057)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£129.50 - £642.50

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Western blot analysis of lysates from MDA-MB-435 cells, using Tau (Phospho-Thr231) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-MAPT-Thr231 antibody (STJ90427)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from rat brain, using Tau (Phospho-Thr534/217) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-MAPT-Thr534 antibody (181-230 aa) (STJ90577)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-MLKL antibody (350-400) (STJ506097)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-Phospho-MLKL antibody (350-400) {Biotin} (STJ506098)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£584.50

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Anti-Phospho-MLKL antibody (350-400) {FITC} (STJ506099)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£584.50

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Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200

Anti-Phospho-MLKL-S358 antibody (STJ193224)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1, phospho-MLKL (S358) Mouse monoclonal antibody (6F8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-Phospho-MLKL-S358 antibody [6F8] (STJ97776)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of extracts of HeLa cells, using Phospho-MLKL-S454 antibody (STJ11102661) at 1:1000 dilution. Hela cells were treated by ATP (5 mM) at 30 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Phospho-MLKL-S454 antibody (STJ11102661)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-Phospho-MLKL-T357/S358/S360 antibody (STJ11101081)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-Phospho-OGT-Thr454 antibody (STJA0009873)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-p53-S20 antibody (STJ11107670)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of extracts of C6 cells, using Phospho-p53-T81 antibody (STJ11103770) at 1:500 dilution. C6 cells were treated by nocodazole (50 ng/ml) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-p53-T81 antibody (STJ11103770)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Western blot analysis of extracts of HeLa cells, using Phospho-PAK2-S20 antibody (STJ117901) at 1:2000 dilution. HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-Phospho-PAK2-S20 antibody (STJ117901)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of C6, using Phospho-PAK2-S20 Rabbit monoclonal antibody (STJ11105893) at 1:2000 dilution. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-PAK2-S20 antibody [S5893RM] (STJ11105893)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from HepG2 cells treated with Adriamycin 0.5uM 24h/Jurkat cells treated with PMA 125ng/ml 30', using PAK2 (Phospho-Ser141) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PAK2-Ser141 antibody (107-156 aa) (STJ90590)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells, using PAK2 (Phospho-Ser192) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PAK2-Ser192 antibody (158-207 aa) (STJ90589)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PAK2 (Phospho-Ser197) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PAK2-Ser197 antibody (163-212 aa) (STJ91214)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 293 cells using Phospho-PAK Gamma (S20) Polyclonal Antibody

Anti-Phospho-PAK2-Ser20 antibody (5-54 aa) (STJ90860)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot of HEK293 cells transfected with Parkin wild type (WT) and Parkin S101 mutant (Mut) showing the specific immunolabeling of the ~52 kDa parkin protein phosphorylated at Ser101.

Anti-Phospho-PARK2-Ser101 antibody (STJA0003742)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Bovine/Non-Human Primates

£454.50

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Western blot of HEK293 cells transfected with Parkin wild type (WT) and Parkin S378 mutant (Mut) showing the specific immunolabeling of the ~52 kDa parkin protein phosphorylated at Ser378.

Anti-Phospho-PARK2-Ser378 antibody (STJA0003743)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Bovine/Non-Human Primates

£454.50

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Western blot analysis of extracts of 293T cells, using Phospho-PKC delta-S645 polyclonal antibody (STJ11103726) at 1:1000 dilution or PKC delta antibody (A0471). 293T cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-PKC delta-S645 antibody (STJ11103726)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunohistochemical analysis of paraffin-embedded human spleen. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PRKCD-Ser359 antibody (STJ196383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-PKC Delta (S645) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-PRKCD-Ser645 antibody (612-661 aa) (STJ91011)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

£48.00 - £252.00

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Western blot analysis of extracts of U-937 cells, using Phospho-PKCdelta-T505 antibody (STJ116396) at 1:2000 dilution. U-937 cells were treated by PMA/TPA (200nM) for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-PRKCD-T505 antibody (STJ116396)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of 3T3 cells using Phospho-PKC Delta (T507) Polyclonal Antibody

Anti-Phospho-PRKCD-Thr507 antibody (471-520 aa) (STJ90388)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HepG2 cells, COLO205 cells and HUVEC cells, using PKC delta (Phospho-Tyr313) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PRKCD-Tyr313 antibody (279-328 aa) (STJ90864)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using PKC delta (Phospho-Tyr52) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PRKCD-Tyr52 antibody (18-67 aa) (STJ91068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 30', using PKCD (Phospho-Tyr64) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PRKCD-Tyr64 antibody (30-79 aa) (STJ90593)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of U937 and Raw264. 7 cells, using Phospho-PKCdelta-Y311 antibody (STJ117870) at 1:1000 dilution. U-937 cells were treated by PMA/TPA (200nM) for 30 minutes. RAW 264. 7 cells were treated by PMA (200 nM) for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-PRKCD-Y311 antibody (STJ117870)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of Jurkat, using Phospho-PRKCQ-S676 antibody (STJ11105774) at 1:1000 dilution. Jurkat cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-PRKCQ-S676 antibody (STJ11105774)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Anti-Phospho-PRKCQ-S695 antibody (STJ11107617)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKC thet (Phospho-Ser676) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PRKCQ-Ser676 antibody (643-692 aa) (STJ90391)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 15', using PKC thet (Phospho-Ser695) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PRKCQ-Ser695 antibody (657-706 aa) (STJ90879)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of Jurkat and HeLa cells, using Phospho-PRKCQ-T538 antibody (STJ117896) at 1:2000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes. HeLa cells were treated by serum-starvation overnight or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-PRKCQ-T538 antibody (STJ117896)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-PRKCQ-Thr538 antibody (504-553 aa) (STJ90865)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 293T AD293 Hela using p-Parkin (S131) antibody. Antibody was diluted at 1:500

Anti-Phospho-PRKN-Ser131 antibody (101-150 aa) (STJ91122)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of 3T3 cells using Phospho-20S Proteasome Alpha 3 (S250) Polyclonal Antibody

Anti-Phospho-PSMA3-Ser250 antibody (206-255 aa) (STJ90556)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western Blot analysis of 1 MCF-7 treated with LPS, 2 MCF7, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-PTK2-Ser722 antibody (STJA0009853)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PTK2-Ser732 antibody (STJ193214)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of HELA KB 293T 3T3 lysis using Phospho-FAK (S843) antibody. Antibody was diluted at 1:1000

Anti-Phospho-PTK2-Ser843 antibody (809-858 aa) (STJ90571)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of HepG2 cells, using FAK (Phospho-Ser910) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PTK2-Ser910 antibody (876-925 aa) (STJ91306)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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