| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Histone H1.4 (1-100) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
H1-4 |
| Gene ID: |
3008 |
| Uniprot ID: |
H14_HUMAN |
| Immunogen Region: |
1-100 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H14 (NP_005312.1). |
| Immunogen Sequence: |
MSETAPAAPAAPAPAEKTPV KKKARKSAGAAKRKASGPPV SELITKAVAASKERSGVSLA ALKKALAAAGYDVEKNNSRI KLGLKSLVSKGTLVQTKGTG |
| Post Translational Modifications | H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter. Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1. Citrullination at Arg-54 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. ADP-ribosylated on Ser-150 in response to DNA damage. |
| Function | Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. |
| Protein Name | Histone H1.4Histone H1bHistone H1s-4 |
| Database Links | Reactome: R-HSA-140342Reactome: R-HSA-2559584 |
| Cellular Localisation | NucleusChromosomeMainly Localizes In HeterochromatinDysplays A Punctuate Staining Pattern In The Nucleus |
| Alternative Antibody Names | Anti-Histone H1.4 antibodyAnti-Histone H1b antibodyAnti-Histone H1s-4 antibodyAnti-H1-4 antibodyAnti-H1F4 antibodyAnti-HIST1H1E antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
