Organelle Biogenesis And Maintenance | Research Area

Western blot analysis of extracts of NIH/3T3 cells, using phospho-STK4-T387 polyclonal antibody (STJ11101048) at 1:1000 dilution or ATF2 antibody (A2155). 293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-ATF2-T69/71 antibody (STJ11101048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of 293T cells, using Phospho-ATF2-T69/T71 antibody (STJ110861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit.

Anti-Phospho-ATF2-T69/T71 antibody (STJ110861)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using Phospho-ATF2-T71 antibody (STJ22025) at 1:1000 dilution. HeLa cells were treated by Anisomycin (25ug/mL) for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by Anisomycin (25ug/mL) for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-ATF2-T71 antibody (STJ22025)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-ATF2-T71 rabbit monoclonal antibody (STJ11102621) at 1:1000 dilution. NIH/3T3 cells were treated by Anisomycin (25 ug/mL) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-Phospho-ATF2-T71 antibody [S1MR] (STJ11102621)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£146.50 - £642.50

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Western blot of UACC903 melanoma cell line lysate (DMSO) showing specific immunolabeling of the ~ 72 kDa ATF2 protein phosphorylated at Thr52. Immunolabeling is reduced by treatment of the lysate with the PKC catalytic inhibitor Gö6850. Image courtesy of Eric Lau and Ze NA ev Ronai.

Anti-Phospho-ATF2-Thr52 antibody (STJA0003584)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£454.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ATF2 (Phospho-Thr69 or 51) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ATF2-Thr69 antibody (36-85 aa) (STJ90185)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-ATF2-Thr69/71 antibody (STJ196245)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-ATF-2 (T71) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-ATF2-Thr71 antibody (38-87 aa) (STJ90186)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-ATF-2 (T73) Polyclonal Antibody

Anti-Phospho-ATF2-Thr73 antibody (40-89 aa) (STJ90187)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using CaMK4 (Phospho-Thr196/200) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CAMK4-Thr200 antibody (166-215 aa) (STJ90203)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of Jurkat cells, using Phospho-PRMT4/CARM1-S228 antibody (STJ22048) at 1:1000 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-CARM1-S228 antibody (STJ22048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of extracts of HT-29 and HeLa cells, using Phospho-CDK1-T14 antibody (STJ22058) at 1:1000 dilution. HT-29 cells were treated by serum-starvation overnight. HeLa cells were treated by UV for 15-30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10min.

Anti-Phospho-CDK1-T14 antibody (STJ22058)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells using Phospho-CDK1-T14 Rabbit monoclonal antibody (STJ11105900) at 1:3000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-CDK1-T14 antibody [S5900RM] (STJ11105900)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of extracts of HeLa cells, using Phospho-CDK1-T161 polyclonal antibody (STJ22061) at 1:1000 dilution or CDK1 antibody (A12414). HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5min.

Anti-Phospho-CDK1-T161 antibody (STJ22061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-CDK1-Thr14 antibody (STJ194705)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-CDK1-Thr161 antibody (126-175 aa) (STJ91199)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-Cdc2 (T161) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-CDK1-Thr161 antibody (131-180 aa) (STJ90213)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-CDK1-Tyr-15 antibody [M231] (STJA0005341)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

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Western blot analysis of Cdc2 (phospho Tyr15) Polyclonal Antibody, using Hela, C6 cell treated or untreated with forskolin 40nM 30', Nocodazole 0.1ug/ml 18hour, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1hour.

Anti-Phospho-CDK1-Tyr15 antibody (5-54 aa) (STJ90214)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of extracts of HT-29 and HeLa cells, using Phospho-CDK1-Y15 antibody (STJ22059) at 1:1000 dilution. HT-29 cells were treated by serum-starvation overnight. HeLa cells were treated by UV for 15-30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-Phospho-CDK1-Y15 antibody (STJ22059)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates, using Phospho-CDK1-Y15 antibody (STJ11105835) at1:2000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-Phospho-CDK1-Y15 antibody [S5835RM] (STJ11105835)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of extracts of 293T cells, using Phospho-CREB1-S129 antibody (STJ11101038) at 1:500 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-CREB1-S129 antibody (STJ11101038)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of HeLa cells, using Phospho-CREB1-S133 polyclonal antibody (STJ22076) at 1:2000 dilution or CREB1 antibody (A11064). HeLa cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-CREB1-S133 antibody (STJ22076)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-CREB1-S133 antibody (STJ22079) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. NIH/3T3 cells were treated by Anisomycin (25 ug/ml) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-CREB1-S133 antibody (STJ22079)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded rat colon using Phospho-CREB1-S133 Rabbit monoclonal antibody (STJ11105877) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-CREB1-S133 antibody [S5877RM] (STJ11105877)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Phospho-CREB1-S142 antibody (STJ11105778)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-Phospho-CREB1-Ser-133 antibody [M415] (STJA0005351)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£319.50

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Anti-Phospho-CREB1-Ser-271 antibody (STJA0005352)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£365.50

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Western blot analysis of lysates from HeLa cells treated with Etoposide 25uM 24h, using CREB (Phospho-Ser121) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CREB1-Ser121 antibody (91-140 aa) (STJ90463)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CREB (Phospho-Ser129) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CREB1-Ser129 antibody (96-145 aa) (STJ90234)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, CREB-1 (phospho Ser133) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-Tubulin monoclonal antibody (5G3) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-CREB1-Ser133 antibody (100-149 aa) (STJ90235)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/IP/ELISA

Reactivity :

Human/Mouse/Rat/Pig

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded Human lung cancer using CREB (Phospho-Ser133) antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-Phospho-CREB1-Ser133 antibody (26-140 aa) [R07-3C4] (STJA0011169)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Rat

£246.50

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Western blot analysis of various cells using Phospho-CREB-1 (S142) Polyclonal Antibody diluted at 1：500

Anti-Phospho-CREB1-Ser142 antibody (111-160 aa) (STJ91016)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HUVEC cells treated with etoposide 25uM 24H, using CREB (Phospho-Thr100) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CREB1-Thr100 antibody (71-120 aa) (STJ90565)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-CRTC1-Ser151 antibody (STJ196456)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Phospho-CRTC2-S171 antibody (STJ11107711)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Immunofluorescence analysis of A549 cells, using HDAC3 (Phospho-Ser424) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-HDAC3-Ser424 antibody (379-428 aa) (STJ91081)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, HDAC6 (phospho Ser22) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Galectin-3 monoclonal antibody (6G2) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-HDAC6-Ser22 antibody (7-56 aa) (STJ91082)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-Phospho-HSP90AA1-Thr5/7 antibody (STJ196324)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western Blot analysis of 1, HeLa cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-MAPK12-Tyr185/182 antibody (STJA0009932)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-MAPK14 antibody (250-300) (STJ502555)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Anti-Phospho-MAPK14 antibody (250-300) {Biotin} (STJ502556)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£584.50

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Anti-Phospho-MAPK14 antibody (250-300) {FITC} (STJ502557)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£584.50

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Immunohistochemistry analysis of paraffin-embedded rat ovary using Phospho-p38 MAPK-Y182 Rabbit polyclonal antibody (STJ11105887) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-MAPK14-T180 antibody (STJ11105887)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunohistochemistry analysis of Phospho-p38 MAPK-T180/Y182 in paraffin-embedded Mouse kidney using Phospho-p38 MAPK-T180/Y182 Rabbit polyclonal antibody (STJ11105812) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-MAPK14-T180/Y182 antibody (STJ11105812)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of Hela cell. 1, p38 (phospho Thr180) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-MAPK14-Thr180 antibody (147-196 aa) (STJ90362)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-MAPK14-Thr180/Y182 antibody (151-200 aa) (STJ90498)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/FC/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Guinea Pig

£48.00 - £252.00

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Western blot analysis of lysates from 1) PC-3, 2) MCF-7 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour. (Red) Tubulin Beta monoclonal antibody (5G3) (STJ96932) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour.

Anti-Phospho-MAPK14-Tyr182 antibody (147-196 aa) (STJ90363)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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