Metabolism Of RNA | Research Area | St John's Laboratory

Immunohistochemistry analysis of paraffin-embedded human kidney using Phospho-Akt-T308 Rabbit monoclonal antibody (STJ11105823) at dilution of 1:60 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-AKT1-T308 antibody [S5823RM] (STJ11105823)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from RD cells, using Phospho-AKT1-T450 Rabbit pAb (STJ22008) at 1:600 dilution. RD cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-AKT1-T450 antibody (STJ22008)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Western blot analysis of lysates from C6 cells, using Phospho-AKT1-T450 Rabbit mAb (STJ11102572) at 1:1000 dilution. C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or treated by PDGF (50 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-AKT1-T450 antibody [S2MR] (STJ11102572)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£118.50 - £521.50

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Anti-Phospho-AKT1-Thr-34 antibody (STJA0005253)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rabbit/Rat

£365.50

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Western blot analysis of various cells using Phospho-Akt1 (T450) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Thr450 antibody (416-465 aa) (STJ90168)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-AKT1-Thr450 antibody [R07-4A7] (STJA0035050)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Rat

£133.50 - £281.50

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Anti-Phospho-AKT1-Thr450 antibody [R72-1U-9] (STJA0035669)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of 3T3 cells using Phospho-Akt1 (T72) Polyclonal Antibody

Anti-Phospho-AKT1-Thr72 antibody (38-87 aa) (STJ90844)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-Akt (Y326) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Tyr326 antibody (292-341 aa) (STJ90503)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-Akt1 (Y474) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Tyr474 antibody (431-480 aa) (STJ90843)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-CCNH-Thr315 antibody (274-323 aa) (STJ90512)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CDK7 (Phospho-Thr170) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CDK7-Thr170 antibody (136-185 aa) (STJ90891)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using CNOT2 (Phospho-Ser101) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CNOT2-Ser101 antibody (67-116 aa) (STJ91265)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using DDX5/DEAD-box Protein 5 (Phospho-Tyr593) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-DDX5-Tyr593 antibody (565-614 aa) (STJ91304)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from HeLa cells, using Phospho-EIF4B-S422 Rabbit pAb (STJ116395) at 1:2000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-EIF4B-S422 antibody (STJ116395)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Anti-Phospho-EIF4B-Ser406 antibody (STJ196297)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-eIF4B-Ser406 antibody [R05-4B2] (STJA0035063)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of 293 cells using Phospho-eIF4B (S422) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-EIF4B-Ser422 antibody (388-437 aa) (STJ90440)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-eIF4E-S209 Rabbit pAb (STJ116377) at 1:500 dilution. NIH/3T3 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-EIF4E-S209 antibody (STJ116377)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-eIF4E-S209 Rabbit mAb (STJ11105791) at 1:1000 dilution. HeLa cells were treated by 20% FBS at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-EIF4E-S209 antibody [S5791RM] (STJ11105791)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£118.50 - £521.50

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Western blot analysis of various cells using Phospho-eIF4E (S209) Polyclonal Antibody

Anti-Phospho-EIF4E-Ser209 antibody (168-217 aa) (STJ90254)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-eIF4E-Ser209 antibody [PT1400R] (STJA0040395)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-eIF4E-Ser209 antibody [R04-5A4] (STJA0034269)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Phospho-eIF4E-Ser209 antibody [R77-8A-2] (STJA0035663)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-EIF4G1-S1108 Rabbit pAb (STJ117894) at 1:1000 dilution. NIH/3T3 cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 60s.

Anti-Phospho-EIF4G1-S1108 antibody (STJ117894)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Phospho-EIF4G1-Ser1108 antibody (STJ196298)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using eIF4G (Phospho-Ser1108) Antibody

Anti-Phospho-EIF4G1-Ser1148 antibody (1074-1123 aa) (STJ91130)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 293 cells using Phospho-hnRNP C1/2 (S260) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-HNRNPC-Ser260 antibody (231-280 aa) (STJ90910)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using hnRPD (Phospho-Ser83) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-HNRNPD-Ser83 antibody (49-98 aa) (STJ91094)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-HNRNPK-Ser216 antibody (STJ90631)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 3T3 cells using Phospho-hnRNP K (S284) Polyclonal Antibody

Anti-Phospho-HNRNPK-Ser284 antibody (250-299 aa) (STJ90527)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Anti-Phospho-Hsp27-Ser78 antibody [R01-8F1] (STJA0036207)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-Hsp27-Ser82 antibody [R04-3X1] (STJA0036052)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Phospho-HSP27/HSPB1-S15 antibody [SRM13690] (STJ11108420)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£0.00

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Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S15 Rabbit pAb (STJ22178) at 1:600 dilution. HeLa cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-HSPB1-S15 antibody (STJ22178)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S78 Rabbit pAb (STJ22181) at 1:1000 dilution. HeLa cells were treated by UV for 15-30 minutes or treated by serum-starvation overnight or treated by Anisomycin (25 Mu g/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-HSPB1-S78 antibody (STJ22181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S78 Rabbit mAb (STJ11102612) at 1:1000 dilution. HeLa cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-HSPB1-S78 antibody [S2MR] (STJ11102612)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of various lysates, primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat： (NA) was diluted at 1:10000, 37°C 1hour.

Anti-Phospho-HSPB1-S78/82 antibody (STJ194697)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S82 Rabbit pAb (STJ22179) at 1:1000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1min.

Anti-Phospho-HSPB1-S82 antibody (STJ22179)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S82 Rabbit mAb (STJ11102611) at 1:1000 dilution. HeLa cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-HSPB1-S82 antibody [S1MR] (STJ11102611)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£118.50 - £521.50

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Western blot analysis of various cells using Phospho-HSP27 (S15) Polyclonal Antibody

Anti-Phospho-HSPB1-Ser15 antibody (5-54 aa) (STJ90294)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-HSP27 (S78) Polyclonal Antibody

Anti-Phospho-HSPB1-Ser78 antibody (45-94 aa) (STJ90295)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-HSP27 (S82) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-HSPB1-Ser82 antibody (48-97 aa) (STJ90296)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-MAPK14 antibody (250-300) (STJ502555)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£497.50

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Anti-Phospho-MAPK14 antibody (250-300) {Biotin} (STJ502556)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£551.50

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Anti-Phospho-MAPK14 antibody (250-300) {FITC} (STJ502557)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£551.50

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Immunohistochemistry analysis of paraffin-embedded rat ovary using Phospho-p38 MAPK-Y182 Rabbit polyclonal antibody (STJ11105887) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-MAPK14-T180 antibody (STJ11105887)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of Hela cell. 1, p38 (phospho Thr180) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-MAPK14-Thr180 antibody (147-196 aa) (STJ90362)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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