Anti-Phospho-HSP27/HSPB1-S15 antibody [SRM13690] (STJ11108420)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide. This information is considered to be commercially sensitive.
General Information
| Short Description | Rabbit monoclonal Phospho-Heat Shock Protein Beta-1-S15 antibody for use in WB, IF, ICC and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM13690 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB, 1:1000-1:5000IF/ICC, 1:50-1:200ELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS containing 50% Glycerol and 0.05% BSA, preserved with proclin300 or Sodium Azide (as specified on the Certificate of Analysis) , pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HSPB1 |
| Gene ID | 3315 |
| Uniprot ID | HSPB1_HUMAN |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Immunogen Sequence | Human |
Additional Info
| Post Translational Modifications | Phosphorylated upon exposure to protein kinase C activators and heat shock. Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement. |
| Function | Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. |
| Protein Name | Heat Shock Protein Beta-1Hspb128 Kda Heat Shock ProteinEstrogen-Regulated 24 Kda ProteinHeat Shock 27 Kda ProteinHsp 27Heat Shock Protein Family B Member 1Stress-Responsive Protein 27Srp27 |
| Database Links | Reactome: R-HSA-4420097Reactome: R-HSA-450408Reactome: R-HSA-5687128Reactome: R-HSA-9009391 |
| Cellular Localisation | CytoplasmNucleusCytoskeletonSpindleCytoplasmic In Interphase CellsColocalizes With Mitotic Spindles In Mitotic CellsTranslocates To The Nucleus During Heat Shock And Resides In Sub-Nuclear Structures Known As Sc35 Speckles Or Nuclear Splicing Speckles |
| Alternative Antibody Names | Anti-Heat Shock Protein Beta-1 antibodyAnti-Hspb1 antibodyAnti-28 Kda Heat Shock Protein antibodyAnti-Estrogen-Regulated 24 Kda Protein antibodyAnti-Heat Shock 27 Kda Protein antibodyAnti-Hsp 27 antibodyAnti-Heat Shock Protein Family B Member 1 antibodyAnti-Stress-Responsive Protein 27 antibodyAnti-Srp27 antibodyAnti-HSPB1 antibodyAnti-HSP27 antibodyAnti-HSP28 antibody |
Information sourced from Uniprot.org